Shotgun Proteomic Analysis of Plasma from Dairy Cattle Suffering from Footrot: Characterization of Potential Disease-Associated Factors

	Shotgun Proteomic Analysis of Plasma from Dairy Cattle Suffering from Footrot: Characterization of Potential Disease-Associated Factors
	Sun, DB; Zhang, H; Guo, DH; Sun, AG; Wang, HB
刊名	PLOS ONE
	2013
卷号	8 期号:2 页码:e55973-e55973
通讯作者	Sun, DB (reprint author), Heilongjiang Bayi Agr Univ, Coll Anim Sci & Vet Med, Daqing, Peoples R China.,dongbosun@yahoo.com.cn ; hbwang@neau.edu.cn
英文摘要	The plasma proteome of healthy dairy cattle and those with footrot was investigated using a shotgun LC-MS/MS approach. In total, 648 proteins were identified in healthy plasma samples, of which 234 were non-redundant proteins and 123 were high-confidence proteins; 712 proteins were identified from footrot plasma samples, of which 272 were non-redundant proteins and 138 were high-confidence proteins. The high-confidence proteins showed significant differences between healthy and footrot plasma samples in molecular weight, isoelectric points and the Gene Ontology categories. 22 proteins were found that may differentiate between the two sets of plasma proteins, of which 16 potential differential expression (PDE) proteins from footrot plasma involved in immunoglobulins, innate immune recognition molecules, acute phase proteins, regulatory proteins, and cell adhesion and cytoskeletal proteins; 6 PDE proteins from healthy plasma involved in regulatory proteins, cytoskeletal proteins and coagulation factors. Of these PDE proteins, haptoglobin, SERPINA10 protein, afamin precursor, haptoglobin precursor, apolipoprotein D, predicted peptidoglycan recognition protein L (PGRP-L) and keratan sulfate proteoglycan (KS-PG) were suggested to be potential footrot-associated factors. The PDE proteins PGRP-L and KS-PG were highlighted as potential biomarkers of footrot in cattle. The resulting protein lists and potential differentially expressed proteins may provide valuable information to increase understanding of plasma protein profiles in cattle and to assist studies of footrot-associated factors.
学科主题	Science & Technology - Other Topics
类目[WOS]	Multidisciplinary Sciences
关键词[WOS]	FUSOBACTERIUM-NECROPHORUM INFECTIONS ; PEPTIDOGLYCAN RECOGNITION PROTEIN ; ACUTE-PHASE RESPONSE ; LARGE GENE LISTS ; KERATAN SULFATE ; HEPATIC-ABSCESS ; OVARIAN-CANCER ; SERUM ; IDENTIFICATION ; BIOMARKER
收录类别	SCI
语种	英语
WOS记录号	WOS:000315970300079
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/329]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Sun, DB,Zhang, H,Guo, DH,et al. Shotgun Proteomic Analysis of Plasma from Dairy Cattle Suffering from Footrot: Characterization of Potential Disease-Associated Factors[J]. PLOS ONE,2013,8(2):e55973-e55973.
APA	Sun, DB,Zhang, H,Guo, DH,Sun, AG,&Wang, HB.(2013).Shotgun Proteomic Analysis of Plasma from Dairy Cattle Suffering from Footrot: Characterization of Potential Disease-Associated Factors.PLOS ONE,8(2),e55973-e55973.
MLA	Sun, DB,et al."Shotgun Proteomic Analysis of Plasma from Dairy Cattle Suffering from Footrot: Characterization of Potential Disease-Associated Factors".PLOS ONE 8.2(2013):e55973-e55973.