Species-specific alternative splicing leads to unique expression of sno-lncRNAs

	Species-specific alternative splicing leads to unique expression of sno-lncRNAs
	Zhang, XO; Yin, QF; Wang, HB; Zhang, Y; Chen, T; Zheng, P; Lu, XH; Chen, LL; Yang, L
刊名	BMC GENOMICS
	2014
卷号	15 期号:1 页码:287-287
关键词	lncRNA sno-lncRNA Alternative splicing Species-specific PWS
通讯作者	Chen, LL (reprint author), Chinese Acad Sci, Inst Biochem & Cell Biol, Shanghai Inst Biol Sci, State Key Lab Mol Biol, Shanghai 200031, Peoples R China.,linglingchen@sibcb.ac.cn ; liyang@picb.ac.cn
英文摘要	Background: Intron-derived long noncoding RNAs with snoRNA ends (sno-lncRNAs) are highly expressed from the imprinted Prader-Willi syndrome (PWS) region on human chromosome 15. However, sno-lncRNAs from other regions of the human genome or from other genomes have not yet been documented. Results: By exploring non-polyadenylated transcriptomes from human, rhesus and mouse, we have systematically annotated sno-lncRNAs expressed in all three species. In total, using available data from a limited set of cell lines, 19 sno-lncRNAs have been identified with tissue-and species-specific expression patterns. Although primary sequence analysis revealed that snoRNAs themselves are conserved from human to mouse, sno-lncRNAs are not. PWS region sno-lncRNAs are highly expressed in human and rhesus monkey, but are undetectable in mouse. Importantly, the absence of PWS region sno-lncRNAs in mouse suggested a possible reason why current mouse models fail to fully recapitulate pathological features of human PWS. In addition, a RPL13A region sno-lncRNA was specifically revealed in mouse embryonic stem cells, and its snoRNA ends were reported to influence lipid metabolism. Interestingly, the RPL13A region sno-lncRNA is barely detectable in human. We further demonstrated that the formation of sno-lncRNAs is often associated with alternative splicing of exons within their parent genes, and species-specific alternative splicing leads to unique expression pattern of sno-lncRNAs in different animals. Conclusions: Comparative transcriptomes of non-polyadenylated RNAs among human, rhesus and mouse revealed that the expression of sno-lncRNAs is species-specific and that their processing is closely linked to alternative splicing of their parent genes. This study thus further demonstrates a complex regulatory network of coding and noncoding parts of the mammalian genome.
学科主题	Biotechnology & Applied Microbiology; Genetics & Heredity
类目[WOS]	Biotechnology & Applied Microbiology ; Genetics & Heredity
关键词[WOS]	LONG NONCODING RNAS ; PRADER-WILLI-SYNDROME ; HUMAN GENOME ; STEM-CELLS ; SEQUENCE ; ALIGNMENT ; GENE ; IDENTIFICATION ; HYPERPHAGIA ; DEFICIENCY
收录类别	SCI
语种	英语
WOS记录号	WOS:000335406900001
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/280]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Zhang, XO,Yin, QF,Wang, HB,et al. Species-specific alternative splicing leads to unique expression of sno-lncRNAs[J]. BMC GENOMICS,2014,15(1):287-287.
APA	Zhang, XO.,Yin, QF.,Wang, HB.,Zhang, Y.,Chen, T.,...&Yang, L.(2014).Species-specific alternative splicing leads to unique expression of sno-lncRNAs.BMC GENOMICS,15(1),287-287.
MLA	Zhang, XO,et al."Species-specific alternative splicing leads to unique expression of sno-lncRNAs".BMC GENOMICS 15.1(2014):287-287.