Crystal structure of the WOPR-DNA complex and implications for Wor1 function in white-opaque switching of Candida albicans

	Crystal structure of the WOPR-DNA complex and implications for Wor1 function in white-opaque switching of Candida albicans
	Zhang, SC; Zhang, TL; Yan, MH; Ding, JP; Chen, JY
刊名	CELL RESEARCH
	2014
卷号	24 期号:9 页码:1108-1120
关键词	crystal structure Wor1 WOPR domain white-opaque switching protein-DNA recognition transcriptional regulation
通讯作者	Chen, JY (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, State Key Lab Mol Biol, 320 Yue Yang Rd, Shanghai 200031, Peoples R China.,jpding@sibcb.ac.cn ; jychen@sibcb.ac.cn
英文摘要	Wor1 (white-opaque switching regulator 1) is a master regulator of the white-opaque switching in Candida albicans, an opportunistic human fungal pathogen, and is associated with its pathogenicity and commensality. Wor1 contains a conserved DNA-binding region at the N-terminus, consisting of two conserved segments (WOPRa and WOPRb) connected by a non-conserved linker that can bind to specific DNA sequences of the promoter regions and then regulates the transcription. Here, we report the crystal structure of the C. albicans Wor1 WOPR segments in complex with a double-stranded DNA corresponding to one promoter region of WOR1. The sequentially separated WOPRa and WOPRb are structurally interwound together to form a compact globular domain that we term the WOPR domain. The WOPR domain represents a new conserved fungal-specific DNA-binding domain which uses primarily a conserved loop to recognize and interact specifically with a conserved 6-bp motif of the DNA in both minor and major grooves. The protein-DNA interactions are essential for WOR1 transcriptional regulation and white-to-opaque switching. The structural and biological data together reveal the molecular basis for the recognition and binding specificity of the WOPR domain with its specific DNA sequences and the function of Wor1 in the activation of transcription.
学科主题	Cell Biology
类目[WOS]	Cell Biology
关键词[WOS]	CONSERVED TRANSCRIPTIONAL REGULATOR ; MASTER REGULATOR ; VIRULENCE ; REQUIRES ; BINDING ; PROTEIN ; SYSTEM
收录类别	SCI
语种	英语
WOS记录号	WOS:000344992900011
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/221]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Zhang, SC,Zhang, TL,Yan, MH,et al. Crystal structure of the WOPR-DNA complex and implications for Wor1 function in white-opaque switching of Candida albicans[J]. CELL RESEARCH,2014,24(9):1108-1120.
APA	Zhang, SC,Zhang, TL,Yan, MH,Ding, JP,&Chen, JY.(2014).Crystal structure of the WOPR-DNA complex and implications for Wor1 function in white-opaque switching of Candida albicans.CELL RESEARCH,24(9),1108-1120.
MLA	Zhang, SC,et al."Crystal structure of the WOPR-DNA complex and implications for Wor1 function in white-opaque switching of Candida albicans".CELL RESEARCH 24.9(2014):1108-1120.