A convenient system for highly specific and sensitive detection of miRNA expression

	A convenient system for highly specific and sensitive detection of miRNA expression
	Li, XQ; Ni, MJ; Zhang, CB; Ma, WB; Zhang, YL
刊名	RNA-A PUBLICATION OF THE RNA SOCIETY
	2014
卷号	20 期号:2 页码:252-259
关键词	miRNA small RNA liquid hybridization avidin-biotin detection system PCR color development
通讯作者	Li, XQ (reprint author), Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, State Key Lab Mol Biol, Shanghai Key Lab Mol Androl, Shanghai 200031, Peoples R China.,lixq@sibs.ac.cn ; ylzhang@sibs.ac.cn
英文摘要	Since the first miRNA was discovered in 1993, miRNAs have become a hotspot for biological research. In order to feed this demand, a robust method is required to detect miRNA gene expression. Development of a detection method is more difficult for miRNAs than for long RNAs, such as mRNA, owing to their small size. Existing methods have limitations; thus, new methods are required. We describe a new system for detecting miRNA expression, which can distinguish miRNA from its precursor and has single-nucleotide resolution. It has single molecule and multiplex detection potential. It may be performed as a polymerase chain reaction (PCR) method, a blotting method, or a macroarray method according to the analyst's preference. This personalized system provides a convenient tool for the detection of miRNA gene expression.
学科主题	Biochemistry & Molecular Biology
类目[WOS]	Biochemistry & Molecular Biology
关键词[WOS]	MICRORNA RESEARCH ; SMALL RNAS ; QUANTIFICATION ; HYBRIDIZATION ; PCR
收录类别	SCI
语种	英语
WOS记录号	WOS:000329850500011
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/150]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Li, XQ,Ni, MJ,Zhang, CB,et al. A convenient system for highly specific and sensitive detection of miRNA expression[J]. RNA-A PUBLICATION OF THE RNA SOCIETY,2014,20(2):252-259.
APA	Li, XQ,Ni, MJ,Zhang, CB,Ma, WB,&Zhang, YL.(2014).A convenient system for highly specific and sensitive detection of miRNA expression.RNA-A PUBLICATION OF THE RNA SOCIETY,20(2),252-259.
MLA	Li, XQ,et al."A convenient system for highly specific and sensitive detection of miRNA expression".RNA-A PUBLICATION OF THE RNA SOCIETY 20.2(2014):252-259.