Andrographolide Prevents High-Fat Diet-Induced Obesity in C57BL/6 Mice by Suppressing the Sterol Regulatory Element-Binding Protein Pathway

	Andrographolide Prevents High-Fat Diet-Induced Obesity in C57BL/6 Mice by Suppressing the Sterol Regulatory Element-Binding Protein Pathway
	Ding, LL; Li, JM; Song, BL; Xiao, X; Huang, WD; Zhang, BF; Tang, XW; Qi, M; Yang, QM; Yang, QL
刊名	JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS
	2014
卷号	351 期号:2 页码:474-483
通讯作者	Yang, L (reprint author), Shanghai Univ Tradit Chinese Med, Inst Tradit Chinese Mat Med, MOE Key Lab Standardizat Chinese Med, Cai Lun Rd 1200, Shanghai 201203, Peoples R China.,yangli7951@hotmail.com ; wangzht@hotmail.com
英文摘要	Sterol regulatory element-binding proteins (SREBPs) are major transcription factors regulating the expression of genes involved in biosynthesis of cholesterol, fatty acids, and triglycerides. We investigated the effect of the specific SREBP suppressor andrographolide, a natural compound isolated from Andrographis paniculata, on the regulation of SREBP signaling by use of Western blot, reporter gene assay, and quantitative real-time polymerase chain reaction analysis. In addition, the antiobesity effects of andrographolide were evaluated in C57BL/6 mice with high-fat diet (HFD)-induced obesity. Our results showed that andrographolide downregulated the expressions of SREBPs target genes and decreased cellular lipid accumulation in vitro. Further, andrographolide (100 mg/kg per day) attenuated HFD-induced body weight gain and fat accumulation in liver or adipose tissues, and improved serum lipid levels and insulin or glucose sensitivity in HFD-induced obese mice. Andrographolide effectively suppressed the respiratory quotient, energy expenditure, and oxygen consumption, which may have contributed to the decreased body-weight gain of the obese mice fed with a HFD. Consistently, andrographolide regulated SREBP target genes and metabolism-associated genes in liver or brown adipose tissue, which may have directly contributed to the lower lipid levels and enhanced insulin sensitivity. Taken together, our results indicated that andrographolide ameliorated lipid metabolism and improved glucose use in mice with HFD-induced obesity. Andrographolide has potential as a leading compound in the prevention or treatment of obesity and insulin resistance.
学科主题	Pharmacology & Pharmacy
类目[WOS]	Pharmacology & Pharmacy
关键词[WOS]	RECEPTOR LXR-ALPHA ; METABOLIC SYNDROME ; X-RECEPTOR ; ADIPOCYTE DIFFERENTIATION ; STATIN INTOLERANCE ; ACID-METABOLISM ; GENE-EXPRESSION ; LDL RECEPTOR ; CHOLESTEROL ; LIVER
收录类别	SCI
语种	英语
WOS记录号	WOS:000348739800017
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/137]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Ding, LL,Li, JM,Song, BL,et al. Andrographolide Prevents High-Fat Diet-Induced Obesity in C57BL/6 Mice by Suppressing the Sterol Regulatory Element-Binding Protein Pathway[J]. JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS,2014,351(2):474-483.
APA	Ding, LL.,Li, JM.,Song, BL.,Xiao, X.,Huang, WD.,...&Wang, ZT.(2014).Andrographolide Prevents High-Fat Diet-Induced Obesity in C57BL/6 Mice by Suppressing the Sterol Regulatory Element-Binding Protein Pathway.JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS,351(2),474-483.
MLA	Ding, LL,et al."Andrographolide Prevents High-Fat Diet-Induced Obesity in C57BL/6 Mice by Suppressing the Sterol Regulatory Element-Binding Protein Pathway".JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS 351.2(2014):474-483.