A G-quadruplex DNA structure resolvase, RHAU, is essential for spermatogonia differentiation

	A G-quadruplex DNA structure resolvase, RHAU, is essential for spermatogonia differentiation
	Gao, X; Ma, W; Nie, J; Zhang, C; Zhang, J; Yao, G; Han, J; Xu, J; Hu, B; Du, Y
刊名	CELL DEATH & DISEASE
	2015
卷号	6 期号:1 页码:e1610-e1610
通讯作者	Zhang, Y (reprint author), Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, State Key Lab Mol Biol, Shanghai Key Lab Mol Androl, 320 Yueyang Rd, Shanghai 200031, Peoples R China.,zhongzhouyang@mail.nju.edu.cn ; xingxuhuang@mail.nju.edu.cn ; ylzhang@sibcb.ac.cn
英文摘要	G-quadruplex (G4) DNA and G4 DNA resolvase are involved in a variety of biological processes. To understand the biological function of G4 DNA structures and their resolvases in spermatogenesis, we investigated the distribution of G4 structures in mouse testis and identified their alterations during spermatogenesis. Meanwhile, we studied the function of RNA helicase associated with AU-rich element (RHAU), a G4 DNA resolvase, in spermatogenesis with a germ-cell-specific knockout mouse model. The results showed that the ablation of RHAU in germ cells caused the increase of G4 structures and thus resulted in the decrease of spermatogonial differentiation. c-kit, a spermatogonia differentiation-related gene, contains two G4 DNA motifs on its promoter. We found its expression was significantly downregulated in RHAU conditional knockout testis. A further analysis demonstrated that RHAU directly bound to the G4 structures to activate c-kit expression. We concluded that RHAU regulates spermatogonia differentiation by promoting c-kit expression via directly binding to the G4 DNA motifs c-kit promoter.
学科主题	Cell Biology
类目[WOS]	Cell Biology
关键词[WOS]	DOUBLE-STRAND BREAKS ; CELL SELF-RENEWAL ; RNA HELICASE RHAU ; C-KIT ONCOGENE ; TETRAMOLECULAR QUADRUPLEX ; PROMOTER REGION ; GENE-EXPRESSION ; REGULATED GENES ; SMALL-MOLECULE ; MAJOR SOURCE
收录类别	SCI
语种	英语
WOS记录号	WOS:000349020500029
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/114]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Gao, X,Ma, W,Nie, J,et al. A G-quadruplex DNA structure resolvase, RHAU, is essential for spermatogonia differentiation[J]. CELL DEATH & DISEASE,2015,6(1):e1610-e1610.
APA	Gao, X.,Ma, W.,Nie, J.,Zhang, C.,Zhang, J.,...&Zhang, Y.(2015).A G-quadruplex DNA structure resolvase, RHAU, is essential for spermatogonia differentiation.CELL DEATH & DISEASE,6(1),e1610-e1610.
MLA	Gao, X,et al."A G-quadruplex DNA structure resolvase, RHAU, is essential for spermatogonia differentiation".CELL DEATH & DISEASE 6.1(2015):e1610-e1610.