Targeting lung cancer stem-like cells with TRAIL gene armed oncolytic adenovirus

	Targeting lung cancer stem-like cells with TRAIL gene armed oncolytic adenovirus
	Yang, Y; Xu, HN; Huang, WD; Ding, M; Xiao, J; Yang, DM; Li, HG; Liu, XY; Chu, L
刊名	JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
	2015
卷号	19 期号:5 页码:915-923
关键词	lung cancer stem-like cells A549 sphere cells oncolytic adenovirus ZD55-TRAIL apoptosis
通讯作者	Chu, L (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, 320 Yue Yang Rd, Shanghai 200031, Peoples R China.,lchu@sibcb.ac.cn
英文摘要	Lung cancer stem cell (LCSC) is critical in cancer initiation, progression, drug resistance and relapse. Disadvantages showed in conventional lung cancer therapy probably because of its existence. In this study, lung cancer cell line A549 cells propagated as spheroid bodies (named as A549 sphere cells) in growth factors-defined serum-free medium. A549 sphere cells displayed CSC properties, including chemo-resistance, increased proportion of G0/G1 cells, slower proliferation rate, ability of differentiation and enhanced tumour formation ability in vivo. Oncolytic adenovirus ZD55 carrying EGFP gene, ZD55-EGFP, infected A549 sphere cells and inhibited cell growth. Tumour necrosis factor-related apoptosis-inducing ligand (TRAIL) armed oncolytic adenovirus, ZD55-TRAIL, exhibited enhanced cytotoxicity and induced A549 sphere cells apoptosis through mitochondrial pathway. Moreover, small molecules embelin, LY294002 and resveratrol improved the cytotoxicity of ZD55-TRAIL. In the A549 sphere cells xenograft models, ZD55-TRAIL significantly inhibited tumour growth and improved survival status of mice. These results suggested that gene armed oncolytic adenovirus is a potential approach for lung cancer therapy through targeting LCSCs.
学科主题	Cell Biology; Research & Experimental Medicine
类目[WOS]	Cell Biology ; Medicine, Research & Experimental
关键词[WOS]	ANTITUMOR-ACTIVITY ; DRUG-RESISTANCE ; IDENTIFICATION ; APOPTOSIS ; PATHWAY ; VIRUS ; STAGE ; GLIOBLASTOMA ; IRRADIATION ; INHIBITORS
收录类别	SCI
语种	英语
WOS记录号	WOS:000353991100002
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/65]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Yang, Y,Xu, HN,Huang, WD,et al. Targeting lung cancer stem-like cells with TRAIL gene armed oncolytic adenovirus[J]. JOURNAL OF CELLULAR AND MOLECULAR MEDICINE,2015,19(5):915-923.
APA	Yang, Y.,Xu, HN.,Huang, WD.,Ding, M.,Xiao, J.,...&Chu, L.(2015).Targeting lung cancer stem-like cells with TRAIL gene armed oncolytic adenovirus.JOURNAL OF CELLULAR AND MOLECULAR MEDICINE,19(5),915-923.
MLA	Yang, Y,et al."Targeting lung cancer stem-like cells with TRAIL gene armed oncolytic adenovirus".JOURNAL OF CELLULAR AND MOLECULAR MEDICINE 19.5(2015):915-923.