RAG1-mediated ubiquitylation of histone H3 is required for chromosomal V(D)J recombination

	RAG1-mediated ubiquitylation of histone H3 is required for chromosomal V(D)J recombination
	Deng, ZM; Liu, HF; Liu, XL
刊名	CELL RESEARCH
	2015
卷号	25 期号:2 页码:181-192
关键词	RAG1 ubiquitylation V(D)J recombination histone H3
通讯作者	Liu, XL (reprint author), Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, State Key Lab Cell Biol, Shanghai 200031, Peoples R China.,liux@sibs.ac.cn
英文摘要	RAG1 and RAG2 proteins are key components in V(D)J recombination. The core region of RAG1 is capable of catalyzing the recombination reaction; however, the biological function of non-core RAG1 remains largely unknown. Here, we show that in a murine-model carrying the RAG1 ring-finger conserved cysteine residue mutation (C325Y), V(D)J recombination was abrogated at the cleavage step, and this effect was accompanied by decreased mono-ubiquitylation of histone H3. Further analyses suggest that un-ubiquitylated histone H3 restrains RAG1 to the chromatin by interacting with the N-terminal 218 amino acids of RAG1. Our data provide evidence for a model in which ubiquitylation of histone H3 mediated by the ring-finger domain of RAG1 triggers the release of RAG1, thus allowing its transition into the cleavage phase. Collectively, our findings reveal that the non-core region of RAG1 facilitates chromosomal V(D)J recombination in a ubiquitylation-dependent pathway.
学科主题	Cell Biology
类目[WOS]	Cell Biology
关键词[WOS]	N-TERMINAL DOMAIN ; UBIQUITIN LIGASE ; RAG1 ; DNA ; CLEAVAGE ; COMPLEX ; PROTEINS ; SEQUENCE ; SIGNAL ; GENES
收录类别	SCI
语种	英语
WOS记录号	WOS:000349334100007
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/22]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Deng, ZM,Liu, HF,Liu, XL. RAG1-mediated ubiquitylation of histone H3 is required for chromosomal V(D)J recombination[J]. CELL RESEARCH,2015,25(2):181-192.
APA	Deng, ZM,Liu, HF,&Liu, XL.(2015).RAG1-mediated ubiquitylation of histone H3 is required for chromosomal V(D)J recombination.CELL RESEARCH,25(2),181-192.
MLA	Deng, ZM,et al."RAG1-mediated ubiquitylation of histone H3 is required for chromosomal V(D)J recombination".CELL RESEARCH 25.2(2015):181-192.