Identification of Two Novel O2-Conotoxins from Conus generalis

	Identification of Two Novel O2-Conotoxins from Conus generalis
	Xu, SQ; Shao, XX; Yan, MD; Chi, CW; Lu, AP; Wang, CG
刊名	INTERNATIONAL JOURNAL OF PEPTIDE RESEARCH AND THERAPEUTICS
	2015
卷号	21 期号:1 页码:81-89
关键词	Conotoxin O2-superfamily cDNA cloning gamma-carboxylation gamma-carboxyglutamic acid Conus generalis
通讯作者	Lu, AP (reprint author), Tongji Univ, Inst Prot Res, Shanghai 200092, Peoples R China.,aplv@tongji.edu.cn ; chunguangwang@tongji.edu.cn
英文摘要	Conus generalis is a type of worm-hunting snail commonly found in subtidal habitats. Fractionation of its venom extract revealed two main peaks overwhelming in the intensity. In this work, by integrating mass spectrometry, N-terminal amino acid sequencing, and molecular cloning, the first dominant fraction was identified as two novel conotoxins, designated ge6b and ge6c, which originate from the same precursor but differ by one gamma-carboxylation modification. These two conotoxins are the first natural peptides identified from C. generalis, and they were classified as members of the O2-superfamily based on the signal sequence. Intracranial injection of the reconstituted fraction into mice induced depressive symptoms, from which the mice recovered after 10-15 min. However, no obvious current change was detected in response to ge6b/c on voltage-gated Na+, K+, or Ca2+ channels, or on nicotinic acetylcholine receptors (nAChR). These results suggest that these two conotoxins may exert their effects through a distinct membrane of channel or receptor.
学科主题	Biochemistry & Molecular Biology
类目[WOS]	Biochemistry & Molecular Biology
关键词[WOS]	GAMMA-CARBOXYGLUTAMIC ACID ; CONOTOXINS ; PEPTIDE ; SUPERFAMILY ; EVOLUTION ; TEXTILE ; VENOMS
收录类别	SCI
语种	英语
WOS记录号	WOS:000348448700011
内容类型	期刊论文
版本	出版稿
源URL	[http://202.127.25.143/handle/331003/6]
专题	上海生化细胞研究所_上海生科院生化细胞研究所
推荐引用方式 GB/T 7714	Xu, SQ,Shao, XX,Yan, MD,et al. Identification of Two Novel O2-Conotoxins from Conus generalis[J]. INTERNATIONAL JOURNAL OF PEPTIDE RESEARCH AND THERAPEUTICS,2015,21(1):81-89.
APA	Xu, SQ,Shao, XX,Yan, MD,Chi, CW,Lu, AP,&Wang, CG.(2015).Identification of Two Novel O2-Conotoxins from Conus generalis.INTERNATIONAL JOURNAL OF PEPTIDE RESEARCH AND THERAPEUTICS,21(1),81-89.
MLA	Xu, SQ,et al."Identification of Two Novel O2-Conotoxins from Conus generalis".INTERNATIONAL JOURNAL OF PEPTIDE RESEARCH AND THERAPEUTICS 21.1(2015):81-89.