Topographical variation in metabolic signatures of human gastrointestinal biopsies revealed by high-resolution magic-angle spinning H-1 NMR spectroscopy

CORC > 武汉物理与数学研究所 > 中国科学院武汉物理与数学研究所 > 2011年以前论文发表（包括2011年）

	Topographical variation in metabolic signatures of human gastrointestinal biopsies revealed by high-resolution magic-angle spinning H-1 NMR spectroscopy
	Wang, Yulan ; Holmes, Elaine ; Comelli, Elena M.; Fotopoulos, Grigorios ; Dorta, Gian ; Tang, Huiru ; Rantalainen, Mattias J.; Lindon, John C.; Corthesy-Theulaz, Irene E.; Fay, Laurent B.
刊名	JOURNAL OF PROTEOME RESEARCH
	2007-10-01
卷号	6 期号:10 页码:3944-3951
关键词	metabonomics antrum duodenum jejunum ileum intestine human pattern recognition biopsy magic-angle spinning NMR spectroscopy
英文摘要	Individual and topographical variation in the metabolic profiles of multiple human gastrointestinal tract (GIT) biopsies have been characterized using high-resolution magic-angle spinning (HRMAS) H-1 NMR spectroscopy and pattern recognition. Samples from antrum, duodenum, jejunum, ileum, and transverse colon were obtained from 8 male and 8 female participants. Each gut region generated a highly characteristic metabolic profile consistent with the varying structural and functional properties of the tissue at different longitudinal levels of the gut. The antral (stomach) mucosa contained higher levels of choline, glycogen, phosphorylethanolamine, and taurine than other gut regions. The spatially close regions of the duodenum and jejunum were equivalent in terms of their gross biochemical composition with high levels of choline, glutathione, glycerophosphocholine (GPC), and lipids relative to other gut regions. The ileal mucosa showed poor discrimination from the duodenum and jejunum tissues and generated strong amino acids signatures but had relative low GPC signals. The colon (large intestine) was high in acetate, glutamate, inositols, and lactate and low in creatine, GPC, and taurine compared to the small intestine. These longitudinal metabolic variations in the human GIT could be attributed to functional variations in energy metabolism, osmoregulation, gut microbial activity, and oxidative protection. This work indicates that 1H HRMAS NMR studies may be of value in analyzing local metabolic variation due to pathological processes in gut biopsies.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biochemical Research Methods
研究领域[WOS]	Biochemistry & Molecular Biology
关键词[WOS]	MAGNETIC-RESONANCE-SPECTROSCOPY ; HUMAN GASTRIC-MUCOSA ; NMR-SPECTROSCOPY ; SMALL-INTESTINE ; BIOMARKER IDENTIFICATION ; PATTERN-RECOGNITION ; BROILER CHICKS ; IN-VITRO ; RAT ; TRACT
收录类别	SCI
语种	英语
WOS记录号	WOS:000249983500009
公开日期	2015-08-18
内容类型	期刊论文
源URL	[http://ir.wipm.ac.cn/handle/112942/4912]
专题	武汉物理与数学研究所_2011年以前论文发表（包括2011年）
作者单位	1.Univ London Imperial Coll Sci Technol & Med, Fac Med, SORA Div, Dept Biomol Med, London SW7 2AZ, England 2.Nestle Res Ctr, Dept Nutr Hlth & Bioanalyt Sci, CH-1000 Lausanne 26, Switzerland 3.Chinese Acad Sci, Wuhan Inst Phys & Math, Wuhan Ctr Magnet Resonance, State Key Lab Magnet Resonance & Atom & Mol Phys, Wuhan 430071, Peoples R China 4.CHU Vaudois, Div Gastroenterol, CH-1011 Lausanne, Switzerland
推荐引用方式 GB/T 7714	Wang, Yulan,Holmes, Elaine,Comelli, Elena M.,et al. Topographical variation in metabolic signatures of human gastrointestinal biopsies revealed by high-resolution magic-angle spinning H-1 NMR spectroscopy[J]. JOURNAL OF PROTEOME RESEARCH,2007,6(10):3944-3951.
APA	Wang, Yulan.,Holmes, Elaine.,Comelli, Elena M..,Fotopoulos, Grigorios.,Dorta, Gian.,...&Nicholson, Jeremy K..(2007).Topographical variation in metabolic signatures of human gastrointestinal biopsies revealed by high-resolution magic-angle spinning H-1 NMR spectroscopy.JOURNAL OF PROTEOME RESEARCH,6(10),3944-3951.
MLA	Wang, Yulan,et al."Topographical variation in metabolic signatures of human gastrointestinal biopsies revealed by high-resolution magic-angle spinning H-1 NMR spectroscopy".JOURNAL OF PROTEOME RESEARCH 6.10(2007):3944-3951.