Analysis of small RNAs derived from Chinese wheat mosaic virus | |
Yang, Jian1; Zheng, Shi-Ling1,2; Zhang, Heng-Mu1; Liu, Xiao-Ya1,3; Li, Jing1; Li, Jun-Min1; Chen, Jian-Ping1 | |
刊名 | ARCHIVES OF VIROLOGY |
2014-11-01 | |
卷号 | 159期号:11页码:3077-3082 |
关键词 | DICER-LIKE PROTEINS PLANT MICRORNAS ARABIDOPSIS FUROVIRUS SEQUENCE IDENTIFICATION POLYMERASES MECHANISM REQUIRES DEFENSE |
ISSN号 | 0304-8608 |
通讯作者 | Zhang, HM (reprint author), Zhejiang Acad Agr Sci, State Key Lab Breeding Base Zhejiang Sustainable, Key Lab Plant Protect & Biotechnol, Minist Agr,Zhejiang Prov Key Lab Plant Virol,Inst, Hangzhou 310021, Zhejiang, Peoples R China. zhhengmu@tsinghua.org.cn ; jpchen2001@yahoo.com.cn |
产权排序 | [Yang, Jian; Zheng, Shi-Ling; Zhang, Heng-Mu; Liu, Xiao-Ya; Li, Jing; Li, Jun-Min; Chen, Jian-Ping] Zhejiang Acad Agr Sci, State Key Lab Breeding Base Zhejiang Sustainable, Key Lab Plant Protect & Biotechnol, Minist Agr,Zhejiang Prov Key Lab Plant Virol,Inst, Hangzhou 310021, Zhejiang, Peoples R China; [Zheng, Shi-Ling] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 304003, Peoples R China; [Liu, Xiao-Ya] Zhejiang Normal Univ, Coll Chem & Life Sci, Jinhua 321004, Peoples R China |
中文摘要 | The virus-derived small interfering RNAs (vsiRNAs) of Chinese wheat mosaic virus (CWMV), a member of the genus Furovirus, were characterised from wheat plants by deep sequencing. CWMV vsiRNAs of 21-22 nt in length predominated, suggesting that there might be a conserved mechanism of DCL2 and DCL4 involvement in the biogenesis of vsiRNAs, as well as a common RNA silencing pathway in CWMV-infected wheat plants. The 5'-terminal base of vsiRNAs was biased towards A/U, suggesting that CWMV vsiRNAs might be loaded into diverse AGO-containing RISCs to disturb the gene expression of host plants. Possible targets for some of the vsiRNAs were predicted. |
英文摘要 | The virus-derived small interfering RNAs (vsiRNAs) of Chinese wheat mosaic virus (CWMV), a member of the genus Furovirus, were characterised from wheat plants by deep sequencing. CWMV vsiRNAs of 21-22 nt in length predominated, suggesting that there might be a conserved mechanism of DCL2 and DCL4 involvement in the biogenesis of vsiRNAs, as well as a common RNA silencing pathway in CWMV-infected wheat plants. The 5'-terminal base of vsiRNAs was biased towards A/U, suggesting that CWMV vsiRNAs might be loaded into diverse AGO-containing RISCs to disturb the gene expression of host plants. Possible targets for some of the vsiRNAs were predicted. |
学科主题 | Virology |
研究领域[WOS] | Virology |
关键词[WOS] | DICER-LIKE PROTEINS ; PLANT MICRORNAS ; ARABIDOPSIS ; FUROVIRUS ; SEQUENCE ; IDENTIFICATION ; POLYMERASES ; MECHANISM ; REQUIRES ; DEFENSE |
收录类别 | SCI |
语种 | 英语 |
WOS记录号 | WOS:000343995900027 |
内容类型 | 期刊论文 |
源URL | [http://ir.yic.ac.cn/handle/133337/8802] |
专题 | 烟台海岸带研究所_中科院烟台海岸带研究所知识产出 |
作者单位 | 1.Zhejiang Acad Agr Sci, State Key Lab Breeding Base Zhejiang Sustainable, Key Lab Plant Protect & Biotechnol, Minist Agr,Zhejiang Prov Key Lab Plant Virol,Inst, Hangzhou 310021, Zhejiang, Peoples R China 2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 304003, Peoples R China 3.Zhejiang Normal Univ, Coll Chem & Life Sci, Jinhua 321004, Peoples R China |
推荐引用方式 GB/T 7714 | Yang, Jian,Zheng, Shi-Ling,Zhang, Heng-Mu,et al. Analysis of small RNAs derived from Chinese wheat mosaic virus[J]. ARCHIVES OF VIROLOGY,2014,159(11):3077-3082. |
APA | Yang, Jian.,Zheng, Shi-Ling.,Zhang, Heng-Mu.,Liu, Xiao-Ya.,Li, Jing.,...&Chen, Jian-Ping.(2014).Analysis of small RNAs derived from Chinese wheat mosaic virus.ARCHIVES OF VIROLOGY,159(11),3077-3082. |
MLA | Yang, Jian,et al."Analysis of small RNAs derived from Chinese wheat mosaic virus".ARCHIVES OF VIROLOGY 159.11(2014):3077-3082. |
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