A Polymeric Liquid Membrane Electrode Responsive to 3,3 ',5,5 '-Tetramethylbenzidine Oxidation for Sensitive Peroxidase/Peroxidase Mimetic-Based Potentiometric Biosensing

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 中科院海岸带环境过程与生态修复重点实验室

	A Polymeric Liquid Membrane Electrode Responsive to 3,3 ',5,5 '-Tetramethylbenzidine Oxidation for Sensitive Peroxidase/Peroxidase Mimetic-Based Potentiometric Biosensing
	Wang, Xuewei 1,2; Yang, Yangang 3; Li, Long 1,2; Sun, Mingshuang 3; Yin, Haogen 3; Qin, Wei1
刊名	ANALYTICAL CHEMISTRY
	2014-05-06
卷号	86 期号:9 页码:4416-4422
关键词	GRAPHENE OXIDE COLORIMETRIC DETERMINATION INJECTION DETERMINATION CATALYTIC-ACTIVITY HYDROGEN-PEROXIDE DNA IMMUNOASSAY BENZIDINE BLOOD NANOPARTICLES
ISSN号	0003-2700
通讯作者	Qin, W (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Key Lab Coastal Environm Proc & Ecol Remediat, Shandong Prov Key Lab Coastal Environm Proc,YICCA, Yantai 264003, Shandong, Peoples R China. wqin@yic.ac.cn
产权排序	[Wang, Xuewei; Li, Long; Qin, Wei] Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Key Lab Coastal Environm Proc & Ecol Remediat, Shandong Prov Key Lab Coastal Environm Proc,YICCA, Yantai 264003, Shandong, Peoples R China; [Wang, Xuewei; Li, Long] Univ Chinese Acad Sci, Beijing 100049, Peoples R China; [Yang, Yangang; Sun, Mingshuang; Yin, Haogen] Yantai Univ, Coll Chem & Chem Engn, Yantai 264003, Shandong, Peoples R China
中文摘要	The oxidation of 3,3',5,5'-tetramethylbenzidine (TMB) has great utility in bioanalysis such as peroxidase/peroxidase mimetic-based biosensing. In this paper, the behaviors of TMB oxidation intermediates/products in liquid/liquid biphasic systems have been investigated for the first time. The free radical, charge transfer complex, and diimine species generated by TMB oxidation are all positively charged under acidic and near-neutral conditions. Electron paramagnetic resonance and visible absorbance spectroscopy data demonstrate that these cationic species can be effectively transferred from an aqueous phase into a water-immiscible liquid phase functionalized by an appropriate cation exchanger. Accordingly, sensitive potential responses of TMB oxidation have been obtained on a cation exchanger-doped polymeric liquid membrane electrode under mildly acidic and near-neutral conditions. By using the membrane electrode responsive to TMB oxidations, two sensitive potentiometric biosensing schemes including the peroxidase-labeled sandwich immunoassay and G-quadruplex DNAzyme-based DNA hybridization assay have been developed. The obtained detection limits for the target antigen and DNA are 0.02 ng/mL and 0.1 nM, respectively. Coupled with other advantages such as low cost, high reliability, and ease of miniaturization and integration, the proposed polymeric liquid membrane electrode holds great promise as a facile and efficient transducer for TMB oxidation and related biosensing applications.
英文摘要	The oxidation of 3,3',5,5'-tetramethylbenzidine (TMB) has great utility in bioanalysis such as peroxidase/peroxidase mimetic-based biosensing. In this paper, the behaviors of TMB oxidation intermediates/products in liquid/liquid biphasic systems have been investigated for the first time. The free radical, charge transfer complex, and diimine species generated by TMB oxidation are all positively charged under acidic and near-neutral conditions. Electron paramagnetic resonance and visible absorbance spectroscopy data demonstrate that these cationic species can be effectively transferred from an aqueous phase into a water-immiscible liquid phase functionalized by an appropriate cation exchanger. Accordingly, sensitive potential responses of TMB oxidation have been obtained on a cation exchanger-doped polymeric liquid membrane electrode under mildly acidic and near-neutral conditions. By using the membrane electrode responsive to TMB oxidations, two sensitive potentiometric biosensing schemes including the peroxidase-labeled sandwich immunoassay and G-quadruplex DNAzyme-based DNA hybridization assay have been developed. The obtained detection limits for the target antigen and DNA are 0.02 ng/mL and 0.1 nM, respectively. Coupled with other advantages such as low cost, high reliability, and ease of miniaturization and integration, the proposed polymeric liquid membrane electrode holds great promise as a facile and efficient transducer for TMB oxidation and related biosensing applications.
学科主题	Chemistry, Analytical
研究领域[WOS]	Chemistry
关键词[WOS]	GRAPHENE OXIDE ; COLORIMETRIC DETERMINATION ; INJECTION DETERMINATION ; CATALYTIC-ACTIVITY ; HYDROGEN-PEROXIDE ; DNA ; IMMUNOASSAY ; BENZIDINE ; BLOOD ; NANOPARTICLES
收录类别	SCI
语种	英语
WOS记录号	WOS:000335719900050
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/8747]
专题	烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室
作者单位	1.Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Key Lab Coastal Environm Proc & Ecol Remediat, Shandong Prov Key Lab Coastal Environm Proc,YICCA, Yantai 264003, Shandong, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Yantai Univ, Coll Chem & Chem Engn, Yantai 264003, Shandong, Peoples R China
推荐引用方式 GB/T 7714	Wang, Xuewei,Yang, Yangang,Li, Long,et al. A Polymeric Liquid Membrane Electrode Responsive to 3,3 ',5,5 '-Tetramethylbenzidine Oxidation for Sensitive Peroxidase/Peroxidase Mimetic-Based Potentiometric Biosensing[J]. ANALYTICAL CHEMISTRY,2014,86(9):4416-4422.
APA	Wang, Xuewei,Yang, Yangang,Li, Long,Sun, Mingshuang,Yin, Haogen,&Qin, Wei.(2014).A Polymeric Liquid Membrane Electrode Responsive to 3,3 ',5,5 '-Tetramethylbenzidine Oxidation for Sensitive Peroxidase/Peroxidase Mimetic-Based Potentiometric Biosensing.ANALYTICAL CHEMISTRY,86(9),4416-4422.
MLA	Wang, Xuewei,et al."A Polymeric Liquid Membrane Electrode Responsive to 3,3 ',5,5 '-Tetramethylbenzidine Oxidation for Sensitive Peroxidase/Peroxidase Mimetic-Based Potentiometric Biosensing".ANALYTICAL CHEMISTRY 86.9(2014):4416-4422.