Potentiometric flow injection sensing system for determination of heparin based on current-controlled release of protamine

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 中科院海岸带环境过程与生态修复重点实验室

	Potentiometric flow injection sensing system for determination of heparin based on current-controlled release of protamine
	Qin, Wei; Ding, Jiawang
刊名	ANALYTICA CHIMICA ACTA
	2015-02-09
卷号	858 页码:60-65
关键词	Potentiometry Polycation-sensitive membrane electrodes Protamine Heparin Current-controlled release Flow injection analysis
ISSN号	0003-2670
通讯作者	Qin, W (reprint author), Chinese Acad Sci, Shandong Prov Key Lab Coastal Environm Proc, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai Inst Coastal Zone Res YIC,YICCAS, Yantai 264003, Shandong, Peoples R China. wqin@yic.ac.cn
产权排序	[Lei, Jiahong; Ding, Jiawang; Chen, Yan; Qin, Wei] Chinese Acad Sci, Shandong Prov Key Lab Coastal Environm Proc, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai Inst Coastal Zone Res YIC,YICCAS, Yantai 264003, Shandong, Peoples R China; [Lei, Jiahong; Ding, Jiawang] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
中文摘要	A flow injection system incorporated with a polycation-sensitive polymeric membrane electrode in the flow cell is proposed for potentiometric determination of heparin. An external current in nano-ampere scale is continuously applied across the polymeric membrane for controlled release of protamine from the inner filling solution to the sample solution, which makes the electrode membrane regenerate quickly after each measurement. The protamine released at membrane-sample interface is consumed by heparin injected into the flow cell via their strong electrostatic interaction, thus decreasing the measured potential, by which heparin can be detected. Under optimized conditions, a linear relationship between the potential peak height and the concentration of heparin in the sample solution can be obtained in the range of 0.1-2.0 U mL (1), and the detection limit is 0.06 U mL (1). The proposed potentiometric sensing system has been successfully applied to the determination of heparin in undiluted sheep whole blood. (C) 2014 Elsevier B.V. All rights reserved.
学科主题	Chemistry, Analytical
研究领域[WOS]	Instrument Developing Project of the Chinese Academy of Sciences [YZ201161] ; National Natural Science Foundation of China [41176081, 21207156] ; Taishan Scholar Program of Shandong Province
关键词[WOS]	SENSITIVE MEMBRANE ELECTRODES ; MOLECULAR-WEIGHT HEPARIN ; POLYION SENSORS ; ELECTROCHEMICAL SENSOR ; RESPONSE MECHANISM ; WHOLE-BLOOD ; LABEL-FREE ; POLYANIONS ; SURGERY
收录类别	SCI
语种	英语
WOS记录号	WOS:000348038600008
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/8655]
专题	烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室烟台海岸带研究所_环境化学实验室
推荐引用方式 GB/T 7714	Qin, Wei,Ding, Jiawang. Potentiometric flow injection sensing system for determination of heparin based on current-controlled release of protamine[J]. ANALYTICA CHIMICA ACTA,2015,858:60-65.
APA	Qin, Wei,&Ding, Jiawang.(2015).Potentiometric flow injection sensing system for determination of heparin based on current-controlled release of protamine.ANALYTICA CHIMICA ACTA,858,60-65.
MLA	Qin, Wei,et al."Potentiometric flow injection sensing system for determination of heparin based on current-controlled release of protamine".ANALYTICA CHIMICA ACTA 858(2015):60-65.