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	Eichhornia crassipes Cleans Wetlands by Enhancing the Nitrogen Removal and Modulating Denitrifying Bacteria Community
	Yi, Neng1,2; Gao, Yan2; Long, Xiao-hua1; Zhang, Zhi-yong2; Guo, Jun-yao2; Shao, Hong-bo1,3; Zhang, Zhen-hua2; Yan, Shao-hua1,2
刊名	CLEAN-SOIL AIR WATER
	2014-05-01
卷号	42期号:5页码:664-673
关键词	Denitrification gene DGGE 15N Stable isotope tracing qPCR Water hyacinth cleaning
ISSN号	1863-0650
通讯作者	Yan, SH (reprint author), Nanjing Agr Univ, Key Lab Marine Biol Jiangsu Prov, Nanjing 210095, Jiangsu, Peoples R China. shaohongbochu@126.com ; shyan@jaas.ac.cn
产权排序	[Yi, Neng; Long, Xiao-hua; Shao, Hong-bo; Yan, Shao-hua] Nanjing Agr Univ, Key Lab Marine Biol Jiangsu Prov, Nanjing 210095, Jiangsu, Peoples R China; [Yi, Neng; Gao, Yan; Zhang, Zhi-yong; Guo, Jun-yao; Zhang, Zhen-hua; Yan, Shao-hua] Jiangsu Acad Agr Sci, Inst Agr Resources & Environm, Nanjng, Peoples R China; [Shao, Hong-bo] Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Key Lab Coastal Biol & Bioresources Utilizat, Yantai, Peoples R China
中文摘要	The role of floating macrophytes on modulating the microbial nitrogen removal is not well understood. In this study, the cultivation of Eichhornia crassipes in eutrophic water may affect the nitrogen (N) fate by modulating the denitrifying bacteria diversity and abundance. The gaseous N losses via denitrification were estimated by 15N stable isotope tracing and the diversity and abundance of denitrifying genes (nirS, nirK, and nosZ) were investigated by molecular tools. The denaturing gradient gel electrophoresis (DGGE) profiles showed that the diversity of denitrifying genes in the treatments with E. crassipes was significantly higher than that in the treatment without E. crassipes. The real-time PCR (qPCR) results showed the trend of denitrifier abundance in the entire system was in the order of N-ER (nitrate with just root of E. crassipes) and A-ER (ammonia with just root of E. crassipes)>N-R (nitrate with E. crassipes) and A-R (ammonia with E. crassipes)>N-W (nitrate without plant) and A-W (ammonia without plant). The gaseous 15N losses via denitrification were significantly and positively related to the abundance of nirK, nirS, and nosZ genes. The results indicated that cultivation of E. crassipes in eutrophic water could increase the diversity and abundance of denitrifying bacteria, resulting in more N being removed as gases via denitrification.
英文摘要	The role of floating macrophytes on modulating the microbial nitrogen removal is not well understood. In this study, the cultivation of Eichhornia crassipes in eutrophic water may affect the nitrogen (N) fate by modulating the denitrifying bacteria diversity and abundance. The gaseous N losses via denitrification were estimated by 15N stable isotope tracing and the diversity and abundance of denitrifying genes (nirS, nirK, and nosZ) were investigated by molecular tools. The denaturing gradient gel electrophoresis (DGGE) profiles showed that the diversity of denitrifying genes in the treatments with E. crassipes was significantly higher than that in the treatment without E. crassipes. The real-time PCR (qPCR) results showed the trend of denitrifier abundance in the entire system was in the order of N-ER (nitrate with just root of E. crassipes) and A-ER (ammonia with just root of E. crassipes)>N-R (nitrate with E. crassipes) and A-R (ammonia with E. crassipes)>N-W (nitrate without plant) and A-W (ammonia without plant). The gaseous 15N losses via denitrification were significantly and positively related to the abundance of nirK, nirS, and nosZ genes. The results indicated that cultivation of E. crassipes in eutrophic water could increase the diversity and abundance of denitrifying bacteria, resulting in more N being removed as gases via denitrification.
学科主题	Environmental Sciences; Marine & Freshwater Biology; Water Resources
研究领域[WOS]	Environmental Sciences & Ecology ; Marine & Freshwater Biology ; Water Resources
关键词[WOS]	CONSTRUCTED WETLANDS ; OXIDE REDUCTASE ; WATER-QUALITY ; SUBMERGED MACROPHYTES ; DENITRIFICATION GENES ; AGRICULTURAL SOIL ; N2O EMISSIONS ; FRESH-WATER ; NOSZ GENES ; NIRK
收录类别	SCI
语种	英语
WOS记录号	WOS:000337514900017
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/8551]
专题	烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室
作者单位	1.Nanjing Agr Univ, Key Lab Marine Biol Jiangsu Prov, Nanjing 210095, Jiangsu, Peoples R China 2.Jiangsu Acad Agr Sci, Inst Agr Resources & Environm, Nanjng, Peoples R China 3.Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Key Lab Coastal Biol & Bioresources Utilizat, Yantai, Peoples R China
推荐引用方式 GB/T 7714	Yi, Neng,Gao, Yan,Long, Xiao-hua,et al. Eichhornia crassipes Cleans Wetlands by Enhancing the Nitrogen Removal and Modulating Denitrifying Bacteria Community[J]. CLEAN-SOIL AIR WATER,2014,42(5):664-673.
APA	Yi, Neng.,Gao, Yan.,Long, Xiao-hua.,Zhang, Zhi-yong.,Guo, Jun-yao.,...&Yan, Shao-hua.(2014).Eichhornia crassipes Cleans Wetlands by Enhancing the Nitrogen Removal and Modulating Denitrifying Bacteria Community.CLEAN-SOIL AIR WATER,42(5),664-673.
MLA	Yi, Neng,et al."Eichhornia crassipes Cleans Wetlands by Enhancing the Nitrogen Removal and Modulating Denitrifying Bacteria Community".CLEAN-SOIL AIR WATER 42.5(2014):664-673.

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