A practical method of Ludox density gradient centrifugation combined with protargol staining for extracting and estimating ciliates in marine sediments

CORC > 海洋研究所 > 中国科学院海洋研究所 > 海洋生物分类与系统演化实验室

	A practical method of Ludox density gradient centrifugation combined with protargol staining for extracting and estimating ciliates in marine sediments
	Xu, Kuidong1 ; Du, Yongfen 1,2; Lei, Yanli1 ; Dai, Renhai 1,2
刊名	EUROPEAN JOURNAL OF PROTISTOLOGY
	2010-11-01
卷号	46 期号:4 页码:263-270
关键词	Protozoa Ludox-QPS method Extraction efficiency Identification Benthic ciliates
英文摘要	Methodological impediments have long been the main problem in estimating the ecological role of marine benthic ciliates. Percoll density centrifugation is currently the most efficient technique for extracting ciliates from fine-grained sediments, while the high cost and low density of Percoll limit its wide application. We developed a protocol of density gradient centrifugation using the cheap sol Ludox HS 40 in combination with the quantitative protargol stain (QPS) to enumerate and identify marine benthic ciliates. The combined Ludox-QPS method involves sample collection and salt reduction, extraction with Ludox centrifugation, and preparation with the QPS technique. The recovery efficiency of Ludox was first tested with azoic sandy and muddy sediments. A 94-100% recovery rate of ciliates was reached. The method was further tested with natural sandy, muddy-sand and muddy sediments. Excellent extraction efficiencies were consistently obtained: an average of 97.6% for ciliates in sand, and 96.9-97.8% for nematodes in the three types of sediments. The high efficiencies indicate that the method allows for simultaneous enumeration of micro- and meiobenthos. Advantages of the new method include: (i) reliable and cost-efficient operation; (ii) appropriate centrifugation for both micro- and meiobenthos; and (iii) applicability to large samples and routine ecological surveys. (C) 2010 Elsevier GmbH. All rights reserved.
收录类别	SCI
语种	英语
WOS记录号	WOS:000286498900003
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/32895]
专题	海洋研究所_海洋生物分类与系统演化实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100039, Peoples R China
推荐引用方式 GB/T 7714	Xu, Kuidong,Du, Yongfen,Lei, Yanli,et al. A practical method of Ludox density gradient centrifugation combined with protargol staining for extracting and estimating ciliates in marine sediments[J]. EUROPEAN JOURNAL OF PROTISTOLOGY,2010,46(4):263-270.
APA	Xu, Kuidong,Du, Yongfen,Lei, Yanli,&Dai, Renhai.(2010).A practical method of Ludox density gradient centrifugation combined with protargol staining for extracting and estimating ciliates in marine sediments.EUROPEAN JOURNAL OF PROTISTOLOGY,46(4),263-270.
MLA	Xu, Kuidong,et al."A practical method of Ludox density gradient centrifugation combined with protargol staining for extracting and estimating ciliates in marine sediments".EUROPEAN JOURNAL OF PROTISTOLOGY 46.4(2010):263-270.