Developmental Changes for the Hemolymph Metabolome of Silkworm (Bombyx mori L.)

CORC > 武汉物理与数学研究所 > 中国科学院武汉物理与数学研究所 > 磁共振应用研究部

	Developmental Changes for the Hemolymph Metabolome of Silkworm (Bombyx mori L.)
	Zhou, Lihong 1,2,5; Li, Huihui 2; Hao, Fuhua2 ; Li, Ning 2; Liu, Xin 1; Wang, Guoliang 5; Wang, Yulan 2,4; Tang, Huiru 2,3
刊名	JOURNAL OF PROTEOME RESEARCH
	2015-05-01
卷号	14 期号:5 页码:2331-2347
关键词	silkworm hemolymph development metabonomics pigments protein biosynthesis
英文摘要	Silkworm (Bombyx mori) is a lepidopteran-holometabolic model organism. To understand its developmental biochemistry, we characterized the larval hemolymph metabonome from the third instar to prepupa stage using H-1 NMR spectroscopy whilst hemolymph fatty acid composition using GC-FID/MS. We unambiguously assigned more than 60 metabolites, among which tyrosine-o-beta-glucuronide, mesaconate, homocarnosine, and picolinate were reported for the first time from the silkworm hemolymph. Phosphorylcholine was the most abundant metabolite in all developmental stages with exception for the periods before the third and fourth molting. We also found obvious developmental dependence for the hemolymph metabonome involving multiple pathways including protein biosyntheses, glycolysis, TCA cycle, the metabolisms of choline amino acids, fatty acids, purines, and pyrimidines. Most hemolymph amino acids had two elevations during the feeding period of the fourth instar and prepupa stage. Trehalose was the major blood sugar before day 8 of the fifth instar, whereas glucose became the major blood sugar after spinning. C16:0, C18:0 and its unsaturated forms were dominant fatty acids in hemolymph. The developmental changes of hemolymph metabonome were associated with dietary nutrient intakes, biosyntheses of cell membrane, pigments, proteins, and energy metabolism. These findings offered essential biochemistry information in terms of the dynamic metabolic changes during silkworm development.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biochemical Research Methods
研究领域[WOS]	Biochemistry & Molecular Biology
关键词[WOS]	CAENORHABDITIS-ELEGANS ; FAT-BODY ; H-1-NMR SPECTROSCOPY ; INSECT HEMOLYMPH ; TOBACCO HORNWORM ; CYNTHIA-RICINI ; MANDUCA-SEXTA ; NMR ; RESPONSES ; PROTEINS
收录类别	SCI
语种	英语
WOS记录号	WOS:000354004700031
公开日期	2015-07-28
内容类型	期刊论文
源URL	[http://ir.wipm.ac.cn/handle/112942/4745]
专题	武汉物理与数学研究所_磁共振应用研究部
作者单位	1.Huazhong Univ Sci & Technol, Coll Life Sci & Technol, Wuhan 430074, Peoples R China 2.Chinese Acad Sci, Key Lab Magnet Resonance Biol Syst, State Key Lab Magnet Resonance & Atom & Mol Phys, Natl Ctr Magnet Resonance Wuhan,Wuhan Inst Phys &, Wuhan 430071, Peoples R China 3.Fudan Univ, State Key Lab Genet Engn, Collaborat Innovat Ctr Genet & Dev, Metabon & Syst Biol Lab,Sch Life Sci, Shanghai 200433, Peoples R China 4.Zhejiang Univ, Collaborat Innovat Ctr Diag & Treatment Infect Di, Hangzhou 310058, Zhejiang, Peoples R China 5.Jianghan Univ, Coll Life Sci, Wuhan 430056, Peoples R China
推荐引用方式 GB/T 7714	Zhou, Lihong,Li, Huihui,Hao, Fuhua,et al. Developmental Changes for the Hemolymph Metabolome of Silkworm (Bombyx mori L.)[J]. JOURNAL OF PROTEOME RESEARCH,2015,14(5):2331-2347.
APA	Zhou, Lihong.,Li, Huihui.,Hao, Fuhua.,Li, Ning.,Liu, Xin.,...&Tang, Huiru.(2015).Developmental Changes for the Hemolymph Metabolome of Silkworm (Bombyx mori L.).JOURNAL OF PROTEOME RESEARCH,14(5),2331-2347.
MLA	Zhou, Lihong,et al."Developmental Changes for the Hemolymph Metabolome of Silkworm (Bombyx mori L.)".JOURNAL OF PROTEOME RESEARCH 14.5(2015):2331-2347.