Mechanokinetics of receptor-ligand interactions in cell adhesion

	Mechanokinetics of receptor-ligand interactions in cell adhesion
	Li N(李宁); Lv SQ(吕守琴); Zhang Y(章燕); Long M(龙勉)
刊名	Acta Mechanica Sinica
	2015-04
通讯作者邮箱	mlong@imech.ac.cn
卷号	31 期号:2 页码:248-258
关键词	Receptor-ligand interactions Selectins beta(2) integrins 2D binding kinetics
ISSN号	0567-7718
通讯作者	Long, M (reprint author), Chinese Acad Sci, Ctr Biomech & Bioengn, Key Lab Micrograv, Natl Micrograv Lab, Beijing 100190, Peoples R China.
产权排序	[Li, Ning; Lu, Shouqin; Zhang, Yan; Long, Mian] Chinese Acad Sci, Ctr Biomech & Bioengn, Key Lab Micrograv, Natl Micrograv Lab, Beijing 100190, Peoples R China; [Li, Ning; Lu, Shouqin; Zhang, Yan; Long, Mian] Chinese Acad Sci, Inst Mech, Beijing Key Lab Engn Construct & Mechanobiol, Beijing 100190, Peoples R China
中文摘要	Receptor-ligand interactions in blood flow are crucial to initiate such biological processes as inflammatory cascade, platelet thrombosis, as well as tumor metastasis. To mediate cell adhesion, the interacting receptors and ligands must be anchored onto two apposing surfaces of two cells or a cell and a substratum, i.e., two-dimensional (2D) binding, which is different from the binding of a soluble ligand in fluid phase to a receptor, i.e., three-dimensional (3D) binding. While numerous works have been focused on 3D kinetics of receptor-ligand interactions in the immune system, 2D kinetics and its regulations have been less understood, since no theoretical framework or experimental assays were established until 1993. Not only does the molecular structure dominate 2D binding kinetics, but the shear force in blood flow also regulates cell adhesion mediated by interacting receptors and ligands. Here, we provide an overview of current progress in 2D binding and regulations, mainly from our group. Relevant issues of theoretical frameworks, experimental measurements, kinetic rates and binding affinities, and force regulations are discussed. A neutrophil undergoes capture and rolling (or tethering) on the endothelium through selectin-PSGL-1 bonds, followed by slow rolling and firm adhesion through the -integrins LFA-1 and Mac-1 as well as intraluminal crawling and transmigration through the endothelium to the inflamed tissue.
分类号	二类
类目[WOS]	Engineering, Mechanical ; Mechanics
研究领域[WOS]	Engineering ; Mechanics
收录类别	SCI ; EI ; CSCD
原文出处	http://dx.doi.org/10.1007/s10409-015-0407-8
语种	英语
WOS记录号	WOS:000355315900011
内容类型	期刊论文
源URL	[http://dspace.imech.ac.cn/handle/311007/54973]
专题	力学研究所_国家微重力实验室
推荐引用方式 GB/T 7714	Li N,Lv SQ,Zhang Y,et al. Mechanokinetics of receptor-ligand interactions in cell adhesion[J]. Acta Mechanica Sinica,2015,31(2):248-258.
APA	Li N,Lv SQ,Zhang Y,&Long M.(2015).Mechanokinetics of receptor-ligand interactions in cell adhesion.Acta Mechanica Sinica,31(2),248-258.
MLA	Li N,et al."Mechanokinetics of receptor-ligand interactions in cell adhesion".Acta Mechanica Sinica 31.2(2015):248-258.