Reversible Loss of N-Acetylaspartate After 15-Min Transient Middle Cerebral Artery Occlusion in Rat: A Longitudinal Study with In Vivo Proton Magnetic Resonance Spectroscopy

CORC > 武汉物理与数学研究所 > 中国科学院武汉物理与数学研究所 > 磁共振基础研究部

	Reversible Loss of N-Acetylaspartate After 15-Min Transient Middle Cerebral Artery Occlusion in Rat: A Longitudinal Study with In Vivo Proton Magnetic Resonance Spectroscopy
	Qian, Junchao 1,2; Qian, Baojin 3; Lei, Hao 1
刊名	NEUROCHEMICAL RESEARCH
	2013
卷号	38 期号:1 页码:208-217
关键词	N-acetylaspartate Magnetic resonance spectroscopy Cerebral ischemia Middle cerebral artery occlusion Rat
英文摘要	It is generally accepted that N-acetylaspartate (NAA) can be used a biochemical marker for assessing neuronal viability/integrity after cerebral ischemia. However, this view has recently been questioned based on observations showing that after a photothrombotic permanent ischemia the acute decline of NAA in the infracted regions, where massive neuronal loss persists, is reversible over time. In this study, we measured the longitudinal changes of NAA and total creatine (Cr) in ischemic rat brain after a 15-min transient middle cerebral artery occlusion (MCAO) by in vivo H-1 magnetic resonance spectroscopy. The results showed that the levels of NAA and total Cr in the ischemic lesion decrease significantly at 1 day post-ichemia, followed by spontaneous recovery to the control levels by 2 weeks and remained stable thereafter up to 16 weeks. The normalization of NAA and total Cr levels was associated histologically with persisted neuronal loss up to 90 % in the ischemic core, and accompanied by marked reactive astrocytic responses occurring with a similar time course. The absolute T-2 relaxation time in the ischemic lesion increased during acute phase, and declined afterwards during subacute and chronic phases of 15-min MCAO. The delayed decreases of T-2 in the ischemic lesion might be associated with deposition of paramagnetic species, such as manganese and iron originated from chronic inflammation, vascular degradation and/or hemorrhagic transformation. The results of this study give further support to the hypothesis that the recovery of NAA after cerebral ischemia might have contributions from reactive glia cells, and caution the use of NAA as a specific neuronal marker during the chronic stage of cerebral ischemia.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biochemistry & Molecular Biology ; Neurosciences
研究领域[WOS]	Biochemistry & Molecular Biology ; Neurosciences & Neurology
关键词[WOS]	NEUROCHEMICAL PROFILE ; REACTIVE ASTROCYTES ; ADULT FOREBRAIN ; FOCAL ISCHEMIA ; BRAIN ; OLIGODENDROCYTES ; METABOLITES ; STROKE ; EXPRESSION ; INFARCTION
收录类别	SCI
语种	英语
WOS记录号	WOS:000312664400021
公开日期	2015-07-14
内容类型	期刊论文
源URL	[http://ir.wipm.ac.cn/handle/112942/968]
专题	武汉物理与数学研究所_磁共振基础研究部
作者单位	1.Chinese Acad Sci, State Key Lab Magnet Resonance & Atom & Mol Phys, Wuhan Inst Phys & Math, Wuhan 430071, Hubei, Peoples R China 2.Chinese Acad Sci, High Field Magnet Lab, Hefei 230031, Peoples R China 3.Anhui Med Univ, Dept Neurol, Anqing Hosp, Anqing 246003, Peoples R China
推荐引用方式 GB/T 7714	Qian, Junchao,Qian, Baojin,Lei, Hao. Reversible Loss of N-Acetylaspartate After 15-Min Transient Middle Cerebral Artery Occlusion in Rat: A Longitudinal Study with In Vivo Proton Magnetic Resonance Spectroscopy[J]. NEUROCHEMICAL RESEARCH,2013,38(1):208-217.
APA	Qian, Junchao,Qian, Baojin,&Lei, Hao.(2013).Reversible Loss of N-Acetylaspartate After 15-Min Transient Middle Cerebral Artery Occlusion in Rat: A Longitudinal Study with In Vivo Proton Magnetic Resonance Spectroscopy.NEUROCHEMICAL RESEARCH,38(1),208-217.
MLA	Qian, Junchao,et al."Reversible Loss of N-Acetylaspartate After 15-Min Transient Middle Cerebral Artery Occlusion in Rat: A Longitudinal Study with In Vivo Proton Magnetic Resonance Spectroscopy".NEUROCHEMICAL RESEARCH 38.1(2013):208-217.