The delivery of thrombi-specific nanoparticles incorporating oligonucleotides into injured cerebrovascular endothelium

CORC > 武汉物理与数学研究所 > 中国科学院武汉物理与数学研究所 > 磁共振基础研究部

	The delivery of thrombi-specific nanoparticles incorporating oligonucleotides into injured cerebrovascular endothelium
	Shi, Wei 1,2; Mei, Heng 1,2; Deng, Jun 1,2; Chen, Chen 1,2; Wang, Huafang 1,2; Guo, Tao 1,2; Zhang, Bo 1,2; Pang, Zhiqing 3; Jiang, Xinguo 3; Wang, Xuxia 4
刊名	BIOMATERIALS
	2013-05-01
卷号	34 期号:16 页码:4128-4136
关键词	Cerebral infarction Gene therapy Nanoparticles Tissue factor
英文摘要	In acute vascular events, the endothelium derived tissue factor (TF) is the trigger of the coagulation cascade. In this study, EGFP-EGF1 protein-conjugated PEG-PLGA nanoparticle was employed as a TF targeting vehicle, the NF-kappa B decoy oligonucleotides (ODNs) was incorporated into it and the resulting EGF1-EGFP-NP-ODNs were evaluated as a vector for therapy of cortex infarction. At 2 h after transfection of TF expressed rat brain capillary endothelial cell, EGF1-EGFP-NP-ODNs was more efficiently internalized and located in the cytoplasm than NP-ODNs. At 4 h and 6 h after administration, ODNs were present in the nuclei and obviously inhibited the TF expression. At 6 h after i.v. administration in vivo, most EGF1-EGFP-NP were accumulated in the embolism vessels, distributed in the damaged endothelial cells and lowered the TF expression. At 24 h after i.v. administration, MR imaging of cortex infarcts were predominantly dwindled. (c) 2013 Elsevier Ltd. All rights reserved.
WOS标题词	Science & Technology ; Technology
类目[WOS]	Engineering, Biomedical ; Materials Science, Biomaterials
研究领域[WOS]	Engineering ; Materials Science
关键词[WOS]	TISSUE FACTOR EXPRESSION ; CONJUGATED PEGYLATED NANOPARTICLES ; PEG-PLA NANOPARTICLES ; BRAIN DELIVERY ; DRUG-DELIVERY ; IN-VIVO ; INTRACEREBRAL HEMORRHAGE ; THROMBOLYTIC THERAPY ; ISCHEMIC-STROKE ; GENE DELIVERY
收录类别	SCI
语种	英语
WOS记录号	WOS:000317322600020
公开日期	2015-07-14
内容类型	期刊论文
源URL	[http://ir.wipm.ac.cn/handle/112942/893]
专题	武汉物理与数学研究所_磁共振基础研究部
作者单位	1.Huazhong Univ Sci & Technol, Tongji Med Coll, Union Hosp, Inst Hematol, Wuhan 430022, Hubei, Peoples R China 2.Minist Educ, Targeted Biotherapy Key Lab, Wuhan 430022, Hubei, Peoples R China 3.Fudan Univ, Sch Pharm, Dept Pharmaceut, Shanghai 200032, Peoples R China 4.Chinese Acad Sci, Wuhan Inst Phys & Math, State Key Lab Magnet Resonance & Atom & Mol Phys, Wuhan 430071, Hubei, Peoples R China
推荐引用方式 GB/T 7714	Shi, Wei,Mei, Heng,Deng, Jun,et al. The delivery of thrombi-specific nanoparticles incorporating oligonucleotides into injured cerebrovascular endothelium[J]. BIOMATERIALS,2013,34(16):4128-4136.
APA	Shi, Wei.,Mei, Heng.,Deng, Jun.,Chen, Chen.,Wang, Huafang.,...&Hu, Yu.(2013).The delivery of thrombi-specific nanoparticles incorporating oligonucleotides into injured cerebrovascular endothelium.BIOMATERIALS,34(16),4128-4136.
MLA	Shi, Wei,et al."The delivery of thrombi-specific nanoparticles incorporating oligonucleotides into injured cerebrovascular endothelium".BIOMATERIALS 34.16(2013):4128-4136.