Integrated Metabonomic-Proteomic Analysis of an Insect-Bacterial Symbiotic System

CORC > 武汉物理与数学研究所 > 中国科学院武汉物理与数学研究所 > 2011年以前论文发表（包括2011年）

	Integrated Metabonomic-Proteomic Analysis of an Insect-Bacterial Symbiotic System
	Wang, Yulan 2; Carolan, James C.3; Hao, FuHua2 ; Nicholson, Jeremy K.4; Wilkinson, Thomas L.3; Douglas, Angela E.1,5
刊名	JOURNAL OF PROTEOME RESEARCH
	2010-03-01
卷号	9 期号:3 页码:1257-1267
关键词	Acyrthosiphon pisum amino acid metabolism aphid differential protein expression metabonome proteome
产权排序	第一
英文摘要	The health of animals, including humans, is dependent on their resident microbiota, but the complexity of the microbial communities makes these associations difficult to study in most animals. Exceptionally, the microbiology of the pea aphid Acyrthosiphon pisum is dominated by a single bacterium Buchnera aphidicola (B. aphidicola). A (1)H NMR-based metabonomic strategy was applied to investigate metabolic profiles of aphids fed on a low essential amino acid diet and treated by antibiotic to eliminate B. aphidicola. In addition, differential gel electrophoresis (DIGE) with mass spectrometry was utilized to determine the alterations of proteins induced by these treatments. We found that these perturbations resulted in significant changes to the abundance of 15 metabolites and 238 proteins. Ten (67%) of the metabolites with altered abundance were amino acids, with nonessential amino acids increased and essential amino acids decreased by both perturbations. Over-represented proteins in the perturbed treatments included catabolic enzymes with roles in amino acid degradation and glycolysis, various cuticular proteins, and a C-type lectin and regucalcin with candidate defensive roles. This analysis demonstrates the central role of essential amino acid production in the relationship and identifies candidate proteins and processes underpinning the function and persistence of the association.
学科主题	波谱分析
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biochemical Research Methods
研究领域[WOS]	Biochemistry & Molecular Biology
关键词[WOS]	APHID ACYRTHOSIPHON-PISUM ; PEA APHID ; BUCHNERA-APHIDICOLA ; GUT MICROBIOTA ; PROTEIN GENES ; DISEASE ; MICROORGANISMS ; SPECTROSCOPY ; ELECTROPHORESIS ; VISUALIZATION
收录类别	SCI
语种	英语
WOS记录号	WOS:000275088100009
内容类型	期刊论文
源URL	[http://ir.wipm.ac.cn/handle/112942/1963]
专题	武汉物理与数学研究所_2011年以前论文发表（包括2011年）
作者单位	1.Cornell Univ, Dept Entomol, Ithaca, NY 14853 USA 2.Chinese Acad Sci, Wuhan Inst Phys & Math, Wuhan Ctr Magnet Resonance, State Key Lab Magnet Resonance & Atom & Mol Phys, Wuhan 430071, Peoples R China 3.Univ Coll Dublin, Sch Biol & Environm Sci, Dublin 2, Ireland 4.Univ London Imperial Coll Sci Technol & Med, Fac Med, Dept Surg & Canc, Dept Biomol Med, London SW7 2AZ, England 5.Univ York, Dept Biol, York YO10 5YW, N Yorkshire, England
推荐引用方式 GB/T 7714	Wang, Yulan,Carolan, James C.,Hao, FuHua,et al. Integrated Metabonomic-Proteomic Analysis of an Insect-Bacterial Symbiotic System[J]. JOURNAL OF PROTEOME RESEARCH,2010,9(3):1257-1267.
APA	Wang, Yulan,Carolan, James C.,Hao, FuHua,Nicholson, Jeremy K.,Wilkinson, Thomas L.,&Douglas, Angela E..(2010).Integrated Metabonomic-Proteomic Analysis of an Insect-Bacterial Symbiotic System.JOURNAL OF PROTEOME RESEARCH,9(3),1257-1267.
MLA	Wang, Yulan,et al."Integrated Metabonomic-Proteomic Analysis of an Insect-Bacterial Symbiotic System".JOURNAL OF PROTEOME RESEARCH 9.3(2010):1257-1267.