Nuclear Magnetic Resonance for Analysis of Metabolite Composition of Escherichia coli

CORC > 武汉物理与数学研究所 > 中国科学院武汉物理与数学研究所 > 2011年以前论文发表（包括2011年）

	Nuclear Magnetic Resonance for Analysis of Metabolite Composition of Escherichia coli
	Ye Yang-Fang 1,2; Zhang Li-Min 1; An Yan-Peng 1; Hao Fu-Hua 1; Tang Hui-Ru 1
刊名	CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
	2011-08-01
卷号	39 期号:8 页码:1186-1194
关键词	Escherichia coli Metabolome Nuclear magnetic resonance
产权排序	第一
英文摘要	To understand the composition of endogenous metabolites of Escherichia coli an important model organism, a series of high-resolution NMR spectroscopic methods were employed to analyze the metabolites of this strain qualitatively and quantitatively. We found that E. coli metabolome was dominated by more than 40 abundant metabolites including amino acids, organic acids, sugars, nucleoside and its derivatives involving many different metabolic pathways. We further found that glucose level was the highest in stationary-phase E. coli while glutamate, betaine, putrescine and ribose-5-phosphate levels were higher than other metabolites. Our results indicated that NMR method was effective to simultaneously detect the abundant bacterial metabolites of different polarity including both the primary and secondary metabolites.
学科主题	波谱学
WOS标题词	Science & Technology ; Physical Sciences
类目[WOS]	Chemistry, Analytical
研究领域[WOS]	Chemistry
关键词[WOS]	MASS-SPECTROMETRY ; METABOLOMICS ; NMR ; QUANTIFICATION ; SPECTROSCOPY ; EXTRACTION ; PHENOTYPE ; STRATEGY ; STRAINS
收录类别	SCI
语种	英语
WOS记录号	WOS:000296599800012
内容类型	期刊论文
源URL	[http://ir.wipm.ac.cn/handle/112942/1925]
专题	武汉物理与数学研究所_2011年以前论文发表（包括2011年）
作者单位	1.Chinese Acad Sci, State Key Lab Magnet Resonance & Atom & Mol Phys, Wuhan Inst Phys & Math, Wuhan 430071, Peoples R China 2.Ningbo Univ, Key Lab Appl Marine Biotechnol, Minist Educ, Ningbo 315211, Zhejiang, Peoples R China
推荐引用方式 GB/T 7714	Ye Yang-Fang,Zhang Li-Min,An Yan-Peng,et al. Nuclear Magnetic Resonance for Analysis of Metabolite Composition of Escherichia coli[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2011,39(8):1186-1194.
APA	Ye Yang-Fang,Zhang Li-Min,An Yan-Peng,Hao Fu-Hua,&Tang Hui-Ru.(2011).Nuclear Magnetic Resonance for Analysis of Metabolite Composition of Escherichia coli.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,39(8),1186-1194.
MLA	Ye Yang-Fang,et al."Nuclear Magnetic Resonance for Analysis of Metabolite Composition of Escherichia coli".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 39.8(2011):1186-1194.