Characterization of the Interaction between the Salmonella Type III Secretion System Tip Protein SipD and the Needle Protein PrgI by Paramagnetic Relaxation Enhancement

CORC > 武汉物理与数学研究所 > 中国科学院武汉物理与数学研究所 > 2011年以前论文发表（包括2011年）

	Characterization of the Interaction between the Salmonella Type III Secretion System Tip Protein SipD and the Needle Protein PrgI by Paramagnetic Relaxation Enhancement
	Rathinavelan, Thenmalarchelvi 1; Tang, Chun 2; De Guzman, Roberto N.1
刊名	JOURNAL OF BIOLOGICAL CHEMISTRY
	2011-02-01
卷号	286 期号:6 页码:4922-4930
产权排序	第二
英文摘要	Many Gram-negative bacteria that cause major diseases and mortality worldwide require the type III secretion system (T3SS) to inject virulence proteins into their hosts and cause infections. A structural component of the T3SS is the needle apparatus, which consists of a base, an external needle, and a tip complex. In Salmonella typhimurium, the external needle is assembled by the polymerization of the needle protein PrgI. On top of this needle sits a tip complex, which is partly formed by the tip protein SipD. How SipD interacts with PrgI during the assembly of the T3SS needle apparatus remains unknown. The central region of PrgI forms an alpha-helical hairpin, whereas SipD has a long central coiled-coil, which is a defining structural feature of other T3SS tip proteins as well. Using NMR paramagnetic relaxation enhancement, we have identified a specific region on the SipD coiled-coil that interacts directly with PrgI. We present a model of how SipD might dock at the tip of the needle based on our paramagnetic relaxation enhancement results, thus offering new insight about the mechanism of assembly of the T3SS needle apparatus.
学科主题	化学
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biochemistry & Molecular Biology
研究领域[WOS]	Biochemistry & Molecular Biology
关键词[WOS]	COILED-COIL ; BURKHOLDERIA-PSEUDOMALLEI ; SHIGELLA-FLEXNERI ; NMR STRUCTURE ; V-ANTIGEN ; IPAD ; VISUALIZATION ; INJECTISOME ; COMPLEX ; DOMAIN
收录类别	SCI
语种	英语
WOS记录号	WOS:000286975700088
内容类型	期刊论文
源URL	[http://ir.wipm.ac.cn/handle/112942/1868]
专题	武汉物理与数学研究所_2011年以前论文发表（包括2011年）
作者单位	1.Univ Kansas, Dept Mol Biosci, Lawrence, KS 66045 USA 2.Chinese Acad Sci, Wuhan Inst Phys & Math, State Key Lab Phys & Atom Mol Phys, Wuhan 430071, Hubei Province, Peoples R China
推荐引用方式 GB/T 7714	Rathinavelan, Thenmalarchelvi,Tang, Chun,De Guzman, Roberto N.. Characterization of the Interaction between the Salmonella Type III Secretion System Tip Protein SipD and the Needle Protein PrgI by Paramagnetic Relaxation Enhancement[J]. JOURNAL OF BIOLOGICAL CHEMISTRY,2011,286(6):4922-4930.
APA	Rathinavelan, Thenmalarchelvi,Tang, Chun,&De Guzman, Roberto N..(2011).Characterization of the Interaction between the Salmonella Type III Secretion System Tip Protein SipD and the Needle Protein PrgI by Paramagnetic Relaxation Enhancement.JOURNAL OF BIOLOGICAL CHEMISTRY,286(6),4922-4930.
MLA	Rathinavelan, Thenmalarchelvi,et al."Characterization of the Interaction between the Salmonella Type III Secretion System Tip Protein SipD and the Needle Protein PrgI by Paramagnetic Relaxation Enhancement".JOURNAL OF BIOLOGICAL CHEMISTRY 286.6(2011):4922-4930.