SINGLE-LASER 3-COLOR IMMUNOLABELING OF A HISTOLOGICAL SECTION BY LASER-SCANNING MICROSCOPY - APPLICATION TO SENILE PLAQUE-RELATED STRUCTURES IN POSTMORTEM HUMAN BRAIN-TISSUE

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	SINGLE-LASER 3-COLOR IMMUNOLABELING OF A HISTOLOGICAL SECTION BY LASER-SCANNING MICROSCOPY - APPLICATION TO SENILE PLAQUE-RELATED STRUCTURES IN POSTMORTEM HUMAN BRAIN-TISSUE
	Uchihara, T.
刊名	Journal of Histochemistry & Cytochemistry
	1995
卷号	43 期号:1 页码:103-106
ISSN号	0022-1554
中文摘要	We describe a method for observing three-color immunofluorescence simultaneously in a histological section under a confocal laser scanning microscope. In this study we investigated the spatial relationship of blood vessels, reactive microglia, and amyloid beta-protein (A beta) deposits in postmortem brain tissue of patients with Alzheimer's disease. HLA-DR was employed as a marker for activated microglia and von Willebrand factor (vWF) as a marker for vascular endothelial cells. HLA-DR was labeled with R-phycoerythrin (R-PE) and vWF with fluorescein isothiocyanate. A beta-protein was immunostained with the tandem conjugate of R-PE and cyanin 5. Three images were obtained serially by scanning a tissue section with a 488-nm laser beam in combination with an appropriate emission filter for each fluorochrome. Overlaying of these three images permitted simultaneous observation of three distinct structures: activated microglia, blood vessels, and A beta deposits. This technique provides an improved way to study the localization of three different antigens in a single tissue section.
语种	英语
公开日期	2015-06-08
内容类型	期刊论文
源URL	[http://ir.xtbg.org.cn/handle/353005/8943]
专题	西双版纳热带植物园_公共技术服务中心主要仪器相关文献_激光共聚焦显微镜
推荐引用方式 GB/T 7714	Uchihara, T.. SINGLE-LASER 3-COLOR IMMUNOLABELING OF A HISTOLOGICAL SECTION BY LASER-SCANNING MICROSCOPY - APPLICATION TO SENILE PLAQUE-RELATED STRUCTURES IN POSTMORTEM HUMAN BRAIN-TISSUE[J]. Journal of Histochemistry & Cytochemistry,1995,43(1):103-106.
APA	Uchihara, T..(1995).SINGLE-LASER 3-COLOR IMMUNOLABELING OF A HISTOLOGICAL SECTION BY LASER-SCANNING MICROSCOPY - APPLICATION TO SENILE PLAQUE-RELATED STRUCTURES IN POSTMORTEM HUMAN BRAIN-TISSUE.Journal of Histochemistry & Cytochemistry,43(1),103-106.
MLA	Uchihara, T.."SINGLE-LASER 3-COLOR IMMUNOLABELING OF A HISTOLOGICAL SECTION BY LASER-SCANNING MICROSCOPY - APPLICATION TO SENILE PLAQUE-RELATED STRUCTURES IN POSTMORTEM HUMAN BRAIN-TISSUE".Journal of Histochemistry & Cytochemistry 43.1(1995):103-106.