Fast Tracking of Co- Localization of Multiple Markers by Using the Nanozoomer Slide Scanner and NDPViewer

CORC > 西双版纳热带植物园 > 中国科学院西双版纳热带植物园 > 公共技术服务中心主要仪器相关文献 > 激光共聚焦显微镜

	Fast Tracking of Co- Localization of Multiple Markers by Using the Nanozoomer Slide Scanner and NDPViewer
	Zheng, P. P.
刊名	Journal of Cellular Physiology
	2014
卷号	229 期号:8 页码:967-973
ISSN号	0021-9541
中文摘要	The detection of co-localization of immunohistochemical markers in tissues or cells requires the application of the confocal laser scanning microscope (CLSM) to multiple immunofluorescence (MIF) stainings. CLSM is operationally sophisticated but requires time-consuming procedures of imaging and reconstruction, and a professional operator is required for manipulation of the microscopic system. Therefore, this technique is less suitable for the examination of many samples in a short time. Moreover, the technique only allows imaging of selected areas of a sample at one time and is not practical for fast panoramic mapping and tracking of whole tissue sections. Here we show a powerful high-throughput and operationally simple histological approach using the Hamamatsu NDP slide scanner (Hamamatsu Nanozoomer 2.0HT) and its viewing platform (NDP.Viewer). The approach not only enables fast mapping and tracking of overlapping spots or regions stained with multiple markers, but also offers panoramic screening of whole tissue sections with fully electronic manipulation for the visualization and analysis of any individual regions. J. Cell. Physiol. 229: 967-973, 2014. (c) 2013 Wiley Periodicals, Inc.
语种	英语
公开日期	2015-06-08
内容类型	期刊论文
源URL	[http://ir.xtbg.org.cn/handle/353005/8812]
专题	西双版纳热带植物园_公共技术服务中心主要仪器相关文献_激光共聚焦显微镜
推荐引用方式 GB/T 7714	Zheng, P. P.. Fast Tracking of Co- Localization of Multiple Markers by Using the Nanozoomer Slide Scanner and NDPViewer[J]. Journal of Cellular Physiology,2014,229(8):967-973.
APA	Zheng, P. P..(2014).Fast Tracking of Co- Localization of Multiple Markers by Using the Nanozoomer Slide Scanner and NDPViewer.Journal of Cellular Physiology,229(8),967-973.
MLA	Zheng, P. P.."Fast Tracking of Co- Localization of Multiple Markers by Using the Nanozoomer Slide Scanner and NDPViewer".Journal of Cellular Physiology 229.8(2014):967-973.