Flow cytometric analysis of Brassica juncea cell and pollen cultures treated with destruxin B, a toxin produced by Alternaria brassicae

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	Flow cytometric analysis of Brassica juncea cell and pollen cultures treated with destruxin B, a toxin produced by Alternaria brassicae
	Sharma, T. R.
刊名	Physiological and Molecular Plant Pathology
	1996
卷号	48 期号:6 页码:379-387
ISSN号	0885-5765
中文摘要	A rapid and reliable flow cytometric method of determining plant cell and pollen grain viability upon treatment with Alternaria brassicae pathotoxin (destruxin B) has been standardized on the basis of light scattering properties of cells of Brassica juncea cv. Kranti stained with fluorescein diacetate. Data on relative fluorescence of cells treated with 60 and 120 mu g destruxin B ml(-1) were collected. The frequency distribution of fluorescence and histogram statistics showed a gradual loss of fluorescence in destruxin B-treated cells. The cell viability was 81% in untreated cells and 39% in 120 mu g destruxin B ml(-1) treated cells after 96 h of culture in the Murashige and Skoog medium. These observations were similar to those obtained by manual counting with a light microscope using Evans blue. Optical sectioning with a confocal laser scanning microscope revealed plasmolysis of destruxin B-treated cells. Viability of pollen grains of B. juncea cv. Kranti treated with destruxin B was also determined on the basis of their relative fluorescence. (C) 1996 Academic Press Limited
语种	英语
公开日期	2015-06-08
内容类型	期刊论文
源URL	[http://ir.xtbg.org.cn/handle/353005/8774]
专题	西双版纳热带植物园_公共技术服务中心主要仪器相关文献_激光共聚焦显微镜
推荐引用方式 GB/T 7714	Sharma, T. R.. Flow cytometric analysis of Brassica juncea cell and pollen cultures treated with destruxin B, a toxin produced by Alternaria brassicae[J]. Physiological and Molecular Plant Pathology,1996,48(6):379-387.
APA	Sharma, T. R..(1996).Flow cytometric analysis of Brassica juncea cell and pollen cultures treated with destruxin B, a toxin produced by Alternaria brassicae.Physiological and Molecular Plant Pathology,48(6),379-387.
MLA	Sharma, T. R.."Flow cytometric analysis of Brassica juncea cell and pollen cultures treated with destruxin B, a toxin produced by Alternaria brassicae".Physiological and Molecular Plant Pathology 48.6(1996):379-387.