Cytomorphological study of soft tissue neoplasms: role of fluorescent immunocytochemistry in diagnosis

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	Cytomorphological study of soft tissue neoplasms: role of fluorescent immunocytochemistry in diagnosis
	Rekhi, B.
刊名	Cytopathology
	2005
卷号	16 期号:5 页码:219-226
ISSN号	0956-5507
中文摘要	Objective: Exact categorization of soft tissue tumours (STTs) on smears requires application of various ancillary techniques. This study was aimed at evaluating the role of fluorescent immunocytochemistry (FICC) in cyto-diagnosis of 30 STT cases. Methods: Thirty cases of soft tissue tumours were included in the present study. All cases were subjected to routine Giemsa and Papanicolaou stain. Extra smears were made and kept for fluorescent immunostaining. A panel of cytoskeletal antibodies, tagged with FITC (Fluorescein isothyocynate), was employed in all these cases. Fluorescent immunostained smears were examined under Zeiss Confocal Laser scanning microscope, using double immunofluorescence (red-green). Finally, all cases were subjected to biopsy and again immunoperoxidase staining. Results: Among the 30 cases in the present study, unaided cytological diagnoses ranged from ' spindle cell ' tumour in four (13.3%) cases, benign and malignant spindle cell tumour in 17 (56.6%) cases, to malignant mesenchymal tumour in nine (30%) cases. FICC helped in further correct categorization of 25/30 (83.3%) cases viz. leiomyoma (three), benign neurogenic tumour (six), schwannoma (one), dermatofibrosarcoma protuberans (three), synovial sarcoma (two), rhabdomyosarcoma (two), malignant fibrous histiocytoma (five) and malignant peripheral nerve sheath tumour (three). Aggressive fibromatosis was found to be a missed diagnosis in two cases. Overall concordance between cyto-diagnosis with FICC, and histopathology results was 83.3% (P < 0.05). Conclusions: Fluorescent immunocytochemistry is a significant ancillary technique for making a rapid and specific diagnosis of STT, as required for their timely management. Incorporation of a wide panel of antibody markers with clinico-cytological correlation is recommended in forming an exact diagnosis in these cases.
语种	英语
公开日期	2015-06-08
内容类型	期刊论文
源URL	[http://ir.xtbg.org.cn/handle/353005/8757]
专题	西双版纳热带植物园_公共技术服务中心主要仪器相关文献_激光共聚焦显微镜
推荐引用方式 GB/T 7714	Rekhi, B.. Cytomorphological study of soft tissue neoplasms: role of fluorescent immunocytochemistry in diagnosis[J]. Cytopathology,2005,16(5):219-226.
APA	Rekhi, B..(2005).Cytomorphological study of soft tissue neoplasms: role of fluorescent immunocytochemistry in diagnosis.Cytopathology,16(5),219-226.
MLA	Rekhi, B.."Cytomorphological study of soft tissue neoplasms: role of fluorescent immunocytochemistry in diagnosis".Cytopathology 16.5(2005):219-226.