Enhanced Production of Fatty Acids and Astaxanthin in Aurantiochytrium sp by the Expression of Vitreoscilla Hemoglobin

CORC > 昆明植物研究所 > 中国科学院昆明植物研究所 > 资源植物与生物技术所级重点实验室

	Enhanced Production of Fatty Acids and Astaxanthin in Aurantiochytrium sp by the Expression of Vitreoscilla Hemoglobin
	Suen, Yung Lee 1; Tang, Hongmei 2,3; Huang, Junchao2 ; Chen, Feng 4
刊名	JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
	2014-12-24
卷号	62 期号:51 页码:12392-12398
关键词	astaxanthin Aurantiochytrium fatty acid Vitreoscilla hemoglobin thraustochytrids
通讯作者	Huang,JC (reprint author),Chinese Acad Sci,Kunming Inst Bot,Kunming,Peoples R China. ; huangjc@mail.kib.ac.cn ; sfchencoe@pku.edu.cn
英文摘要	Dissolved oxygen is a critical factor for heterotrophic cell growth and metabolite production. The aim of this study was to investigate the effects of an oxygen-involved protein on cell growth and fatty acid and astaxanthin production in the biologically important thraustochytrid Aurantiochytrium sp. The hemoglobin of the Vitreoscilla stercoraria (VHb) gene was fused upstream with a zeocin resistance gene (ble) and driven by the Aurantiochytrium tubulin promoter. The expression construct was introduced into two strains of Aurantiochytrium sp. by electroporation. Transgenic Aurantiochytrium sp. strains MP4 and SK4 expressing the heterologous VHb achieved significantly higher maximum biomass than their corresponding controls in microaerobic conditions. Furthermore, the transformants of Aurantiochytrium sp. SK4 produced 44% higher total fatty acid and 9-fold higher astaxanthin contents than the wild type control in aerobic conditions. The present study highlights the biotechnological application of VHb in high-cell density fermentation for enhanced biomass production as well as high-value metabolites.
学科主题	Agriculture, Multidisciplinary; Chemistry, Applied; Food Science & Technology
类目[WOS]	Agriculture, Multidisciplinary ; Chemistry, Applied ; Food Science & Technology
研究领域[WOS]	Agriculture ; Chemistry ; Food Science & Technology
关键词[WOS]	GENE PHYLOGENY THRAUSTOCHYTRIACEAE ; TAXONOMIC REARRANGEMENT ; SCHIZOCHYTRIUM ; OPTIMIZATION ; XANTHOPHYLLS ; EMENDATION ; MORPHOLOGY ; ERECTION ; SQUALENE ; NOV
收录类别	SCI
语种	英语
WOS记录号	WOS:000347138500015
内容类型	期刊论文
源URL	[http://ir.kib.ac.cn/handle/151853/19376]
专题	昆明植物研究所_资源植物与生物技术所级重点实验室
作者单位	1.Univ Hong Kong,Pokfulam, Sch Biol Sci, Hong Kong, Hong Kong, Peoples R China 2.Chinese Acad Sci, Kunming Inst Bot, Kunming, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China 4.Peking Univ, Coll Engn, Inst Food & Bioresource Engn, Beijing 100871, Peoples R China
推荐引用方式 GB/T 7714	Suen, Yung Lee,Tang, Hongmei,Huang, Junchao,et al. Enhanced Production of Fatty Acids and Astaxanthin in Aurantiochytrium sp by the Expression of Vitreoscilla Hemoglobin[J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,2014,62(51):12392-12398.
APA	Suen, Yung Lee,Tang, Hongmei,Huang, Junchao,&Chen, Feng.(2014).Enhanced Production of Fatty Acids and Astaxanthin in Aurantiochytrium sp by the Expression of Vitreoscilla Hemoglobin.JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,62(51),12392-12398.
MLA	Suen, Yung Lee,et al."Enhanced Production of Fatty Acids and Astaxanthin in Aurantiochytrium sp by the Expression of Vitreoscilla Hemoglobin".JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 62.51(2014):12392-12398.