The comparative proteomics analysis revealed the modulation of inducible nitric oxide on the immune response of scallop Chlamys farreri

CORC > 海洋研究所 > 中国科学院海洋研究所 > 实验海洋生物学重点实验室

	The comparative proteomics analysis revealed the modulation of inducible nitric oxide on the immune response of scallop Chlamys farreri
	Sun, Zhibin 1; Jiang, Qiufen 1,2; Wang, Lingling 1; Zhou, Zhi 1; Wang, Mengqiang1 ; Yi, Qilin 1,2; Song, Linsheng 1
刊名	FISH & SHELLFISH IMMUNOLOGY
	2014-10-01
卷号	40 期号:2 页码:584-594
关键词	Chlamys farreri Immune response Proteome Nitric oxide (NO) Lipopolysaccharide (LPS)
通讯作者	Wang, LL (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China.
英文摘要	Nitric oxide (NO) is an important gasotransmitter which plays a key role on the modulation of immune response in all vertebrates and invertebrates. In the present study, the modulation of inducible NO on immune response of scallop Chlamys farreri was investigated via proteomic analysis. Total proteins from hepatopancreas of scallops treated with lipopolysaccharide (LPS) and/or the inhibitor of vertebrate inducible NO synthase (S-methylisothiourea sulfate, SMT) for 12 h were analyzed via 2-D PAGE and ImageMaster 2D Platinum. There were 890, 1189 and 1046 protein spots detected in the groups treated by phosphate buffered saline (PBS), LPS and LPS+SMT, respectively, and 26 differentially expressed protein spots were identified among them. These proteins were annotated with binding or catalytic activity, and most of them were involved in metabolic or cellular processes. Some immune-related or antioxidant-related molecules such as single Ig IL-1-related receptor, guanine nucleotide-binding protein subunit beta-like protein and peroxiredoxin were identified, and the changes of their expression levels in LPS group were intensified significantly after adding SMT. The decreased expression level of tyrosinase and increased level of glutathione S-transferase 4 in LPS group were diametrically reversed by appending SMT. Moreover, interferon stimulated exonuclease gene 20-like protein and copper chaperone for superoxide dismutase were only induced by LPS+SMT stimulation but not by LPS stimulation. These data indicated that NO could modulate many immunity processes in scallop, such as NF-kappa B transactivation, cytoskeleton reorganization and other pivotal processes, and it was also involved in the energy metabolism, posttranslational modification, detoxification and redox balance during the immune response. (C) 2014 Elsevier Ltd. All rights reserved.
学科主题	Fisheries; Immunology; Marine & Freshwater Biology; Veterinary Sciences
收录类别	SCI
语种	英语
WOS记录号	WOS:000343381100030
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/24174]
专题	海洋研究所_实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Sun, Zhibin,Jiang, Qiufen,Wang, Lingling,et al. The comparative proteomics analysis revealed the modulation of inducible nitric oxide on the immune response of scallop Chlamys farreri[J]. FISH & SHELLFISH IMMUNOLOGY,2014,40(2):584-594.
APA	Sun, Zhibin.,Jiang, Qiufen.,Wang, Lingling.,Zhou, Zhi.,Wang, Mengqiang.,...&Song, Linsheng.(2014).The comparative proteomics analysis revealed the modulation of inducible nitric oxide on the immune response of scallop Chlamys farreri.FISH & SHELLFISH IMMUNOLOGY,40(2),584-594.
MLA	Sun, Zhibin,et al."The comparative proteomics analysis revealed the modulation of inducible nitric oxide on the immune response of scallop Chlamys farreri".FISH & SHELLFISH IMMUNOLOGY 40.2(2014):584-594.