Draft Sequencing and Analysis of the Genome of Pufferfish Takifugu flavidus

CORC > 海洋研究所 > 中国科学院海洋研究所 > 实验海洋生物学重点实验室

	Draft Sequencing and Analysis of the Genome of Pufferfish Takifugu flavidus
	Gao, Yang1,2 ; Gao, Qiang 1; Zhang, Huan 1; Wang, Lingling 1; Zhang, Fuchong 3; Yang, Chuanyan 1; Song, Linsheng 1
刊名	DNA RESEARCH
	2014-12-01
卷号	21 期号:6 页码:627-637
关键词	Takifugu flavidus draft genome NGS
通讯作者	Song, LS (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Shandong, Peoples R China.
英文摘要	The pufferfish Takifugu flavidus is an important economic species due to its outstanding flavour and high market value. It has been regarded as an excellent model of genetic study for decades as well. In the present study, three mate-pair libraries of T. flavidus genome were sequenced by the SOLiD 4 next-generation sequencing platform, and the draft genome was constructed with the short reads using an assisted assembly strategy. The draft consists of 50,947 scaffolds with an N50 value of 305.7 kb, and the average GC content was 45.2%. The combined length of repetitive sequences was 26.5 Mb, which accounted for 6.87% of the genome, indicating that the compactness of T. flavidus genome was approximative with that of T. rubripes genome. A total of 1,253 non-coding RNA genes and 30,285 protein-encoding genes were assigned to the genome. There were 132,775 and 394 presumptive genes playing roles in the colour pattern variation, the relatively slow growth and the lipid metabolism, respectively. Among them, genes involved in the microtubule-dependent transport system, angiogenesis, decapentaplegic pathway and lipid mobilization were significantly expanded in the T. flavidus genome. This draft genome provides a valuable resource for understanding and improving both fundamental and applied research with pufferfish in the future.
学科主题	Genetics & Heredity
收录类别	SCI
语种	英语
WOS记录号	WOS:000347101100005
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/24106]
专题	海洋研究所_实验海洋生物学重点实验室海洋研究所_海洋腐蚀与防护研究发展中心
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Shandong, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Ocean & Fishery Sci Res Inst Hebei Prov, Qinhuangdao 066200, Hebei, Peoples R China
推荐引用方式 GB/T 7714	Gao, Yang,Gao, Qiang,Zhang, Huan,et al. Draft Sequencing and Analysis of the Genome of Pufferfish Takifugu flavidus[J]. DNA RESEARCH,2014,21(6):627-637.
APA	Gao, Yang.,Gao, Qiang.,Zhang, Huan.,Wang, Lingling.,Zhang, Fuchong.,...&Song, Linsheng.(2014).Draft Sequencing and Analysis of the Genome of Pufferfish Takifugu flavidus.DNA RESEARCH,21(6),627-637.
MLA	Gao, Yang,et al."Draft Sequencing and Analysis of the Genome of Pufferfish Takifugu flavidus".DNA RESEARCH 21.6(2014):627-637.