Biological identification and determination of optimum growth conditions for four species of Navicula

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	Biological identification and determination of optimum growth conditions for four species of Navicula
	Zhao Xiaobo 1,2; Pang Shaojun 1; Liu Feng 1; Shan Tifeng 1; Li Jing1,2
刊名	ACTA OCEANOLOGICA SINICA
	2014-08-01
卷号	33 期号:8 页码:111-118
关键词	Navicula SSU rbcL culture conditions
通讯作者	Pang, SJ (reprint author), Chinese Acad Sci, Algal Culture Collect Ctr, Key Lab Expt Marine Biol, Inst Oceanol, Qingdao 266071, Peoples R China.
英文摘要	Four species in the genus Navicula were isolated using the serial dilution method. Based on scanning electron microscopy (SEM) and sequence comparisons of two segments of genes (small ribosomal subunit and large subunit of Rubisco), the species were identified as Navicula perminuta, N. pseudacceptata, N. vara, and N. rhynchocephala. Based on phylogenetic analysis and culture trials, there was a close relationship between N. perminuta and N. vara. Growth of these species was evaluated using measurements of optical density at 680 nm (OD680) under various environmental factors. Results showed that the optimum culture conditions were 25 degrees C, 50-100 mol photons m(-2) s(-1), pH 8.0, and salinities from 25 to 30. However, the favorable salinity for N. perminuta was surprisingly high at 35. Nutrient requirement analysis demonstrated that growth of Navicula depended on the availability of SiO32-. Their relative growth rates (RGR) peaked at the highest tested level (0.25 mmol/L). The optimal concentrations of NO3- and PO43- were 3.6 mmol/L and 0.18 mmol/L, respectively. Culture of these Navicula species for abalone or sea cucumber aquaculture should take these factors into consideration.
学科主题	Oceanography
收录类别	SCI
语种	英语
WOS记录号	WOS:000339318800013
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/24078]
专题	海洋研究所_实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Algal Culture Collect Ctr, Key Lab Expt Marine Biol, Inst Oceanol, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Zhao Xiaobo,Pang Shaojun,Liu Feng,et al. Biological identification and determination of optimum growth conditions for four species of Navicula[J]. ACTA OCEANOLOGICA SINICA,2014,33(8):111-118.
APA	Zhao Xiaobo,Pang Shaojun,Liu Feng,Shan Tifeng,&Li Jing.(2014).Biological identification and determination of optimum growth conditions for four species of Navicula.ACTA OCEANOLOGICA SINICA,33(8),111-118.
MLA	Zhao Xiaobo,et al."Biological identification and determination of optimum growth conditions for four species of Navicula".ACTA OCEANOLOGICA SINICA 33.8(2014):111-118.