Development of an efficient process intensification strategy for enhancing Pfu DNA polymerase production in recombinant Escherichia coli | |
Hu, Jian-Hua1,2; Wang, Feng1; Liu, Chun-Zhao1 | |
刊名 | BIOPROCESS AND BIOSYSTEMS ENGINEERING |
2015-04-01 | |
卷号 | 38期号:4页码:651-659 |
关键词 | Cell growth Escherichia coli Pfu DNA polymerase IPTG induction |
ISSN号 | 1615-7591 |
通讯作者 | Liu, CZ (reprint author), Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China. |
英文摘要 | An efficient induction strategy that consisted of multiple additions of small doses of isopropyl-beta-D-thiogalactopyranoside (IPTG) in the early cell growth phase was developed for enhancing Pfu DNA polymerase production in Escherichia coli. In comparison to the most commonly used method of a single induction of 1 mM IPTG, the promising induction strategy resulted in an increase in the Pfu activity of 13.5 % in shake flasks, while simultaneously decreasing the dose of IPTG by nearly half. An analysis of the intracellular IPTG concentrations indicated that the cells need to maintain an optimum intracellular IPTG concentration after 6 h for efficient Pfu DNA polymerase production. A significant increase in the Pfu DNA polymerase activity of 31.5 % under the controlled dissolved oxygen concentration of 30 % in a 5 L fermentor was achieved using the multiple IPTG induction strategy in comparison with the single IPTG induction. The induction strategy using multiple inputs of IPTG also avoided over accumulation of IPTG and reduced the cost of Pfu DNA polymerase production. |
学科主题 | Biotechnology & Applied Microbiology ; Engineering |
WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Technology |
类目[WOS] | Biotechnology & Applied Microbiology ; Engineering, Chemical |
研究领域[WOS] | Biotechnology & Applied Microbiology ; Engineering |
关键词[WOS] | FED-BATCH CULTURES ; PYROCOCCUS-FURIOSUS ; E. COLI ; EXPRESSION ; PROTEIN ; OXYGEN ; IPTG ; ACCUMULATION ; PURIFICATION ; LIMITATION |
收录类别 | SCI |
语种 | 英语 |
WOS记录号 | WOS:000351513500005 |
内容类型 | 期刊论文 |
源URL | [http://ir.ipe.ac.cn/handle/122111/13794] |
专题 | 过程工程研究所_研究所(批量导入) |
作者单位 | 1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
推荐引用方式 GB/T 7714 | Hu, Jian-Hua,Wang, Feng,Liu, Chun-Zhao. Development of an efficient process intensification strategy for enhancing Pfu DNA polymerase production in recombinant Escherichia coli[J]. BIOPROCESS AND BIOSYSTEMS ENGINEERING,2015,38(4):651-659. |
APA | Hu, Jian-Hua,Wang, Feng,&Liu, Chun-Zhao.(2015).Development of an efficient process intensification strategy for enhancing Pfu DNA polymerase production in recombinant Escherichia coli.BIOPROCESS AND BIOSYSTEMS ENGINEERING,38(4),651-659. |
MLA | Hu, Jian-Hua,et al."Development of an efficient process intensification strategy for enhancing Pfu DNA polymerase production in recombinant Escherichia coli".BIOPROCESS AND BIOSYSTEMS ENGINEERING 38.4(2015):651-659. |
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