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	The brighter side of soils Quantum dots track organic nitrogen through fungi and plants
	Whiteside, M. D.
刊名	Ecology
	2009
卷号	90期号:1页码:100-108
ISSN号	0012-9658
中文摘要	Soil microorganisms mediate many nutrient transformations that are central in terrestrial cycling of carbon and nitrogen. However, uptake of organic nutrients by microorganisms is difficult to study in natural systems. We assessed quantum dots (fluorescent nanoscale semiconductors) as a new tool to observe uptake and translocation of organic nitrogen by fungi and plants. We conjugated quantum dots to the amino groups of glycine, arginine, and chitosan and incubated them with Penicillium fungi (a saprotroph) and annual bluegrass (Poa annua) inoculated with arbuscular mycorrhizal fungi. As experimental controls, we incubated fungi and bluegrass samples with substrate-free quantum dots as well as unbound quantum dot substrate mixtures. Penicillium fungi, annual bluegrass, and arbuscular mycorrhizal fungi all showed uptake and translocation of quantum dot-labeled organic nitrogen, but no uptake of quantum dot controls. Additionally, we observed quantum dot-labeled organic nitrogen within soil hyphae, plant roots, and plant shoots using field imaging techniques. This experiment is one of the first to demonstrate direct uptake of organic nitrogen by arbuscular mycorrhizal fungi.
语种	英语
公开日期	2015-04-03
内容类型	期刊论文
源URL	[http://ir.xtbg.org.cn/handle/353005/8558]
专题	西双版纳热带植物园_公共技术服务中心主要仪器相关文献_植物活体成像系统
推荐引用方式 GB/T 7714	Whiteside, M. D.. The brighter side of soils Quantum dots track organic nitrogen through fungi and plants[J]. Ecology,2009,90(1):100-108.
APA	Whiteside, M. D..(2009).The brighter side of soils Quantum dots track organic nitrogen through fungi and plants.Ecology,90(1),100-108.
MLA	Whiteside, M. D.."The brighter side of soils Quantum dots track organic nitrogen through fungi and plants".Ecology 90.1(2009):100-108.

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