Gold Nanoparticle-Based Enzyme-Linked Antibody-Aptamer Sandwich Assay for Detection of Salmonella Typhimurium | |
Wu, WH ; Li, J ; Pan, D ; Li, J ; Song, SP ; Rong, MG ; Li, ZX ; Gao, JM ; Lu, JX | |
刊名 | ACS APPLIED MATERIALS & INTERFACES
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2014 | |
卷号 | 6期号:19页码:16974—16981 |
关键词 | MAGNETOELASTIC BIOSENSORS ELISA BINDING IMMUNOASSAY SELECTION MOLECULE SEQUENCE |
ISSN号 | 1944-8244 |
通讯作者 | jxlu313@163.com |
英文摘要 | Enzyme-linked immunosorbent assay (ELISA) provides a convenient means for the detection of Salmonella enterica serovar Typhimurium (STM), which is important for rapid diagnosis of foodborne pathogens. However, conventional ELISA is limited by antibody-antigen immunoreactions and suffers from poor sensitivity and tedious sample pretreatment. Therefore, development of novel ELISA remains challenging. Herein, we designed a comprehensive strategy for rapid, sensitive, and quantitative detection of STM with high specificity by gold nanoparticle-based enzyme-linked antibody-aptamer sandwich (nano-ELAAS) method. STM was captured and preconcentrated from samples with aptamer-modified magnetic particles, followed by binding with detector antibodies. Then nanoprobes carrying a large amount of reporter antibodies and horseradish peroxidase molecules were used for colorimetric signal amplification. Under the optimized reaction conditions, the nano-ELAAS assay had a quantitative detection range from 1 X 10(3) to 1 X 10(8) CFU mL(-1), a limit of detection of 1 X 10(3) CFU mL(-1), and a selectivity of >10-fold for STM in samples containing other bacteria at higher concentration with an assay time less than 3 h. In addition, the developed nanoprobes were improved in terms of detection range and/or sensitivity when compared with two commercial enzyme-labeled antibody signal reporters. Finally, the nano-ELAAS method was demonstrated to work well in milk samples, a common source of STM contamination. |
收录类别 | SCI |
语种 | 英语 |
WOS记录号 | WOS:000343018200061 |
公开日期 | 2015-03-13 |
内容类型 | 期刊论文 |
源URL | [http://ir.sinap.ac.cn/handle/331007/14164] ![]() |
专题 | 上海应用物理研究所_中科院上海应用物理研究所2011-2017年 |
推荐引用方式 GB/T 7714 | Wu, WH,Li, J,Pan, D,et al. Gold Nanoparticle-Based Enzyme-Linked Antibody-Aptamer Sandwich Assay for Detection of Salmonella Typhimurium[J]. ACS APPLIED MATERIALS & INTERFACES,2014,6(19):16974—16981. |
APA | Wu, WH.,Li, J.,Pan, D.,Li, J.,Song, SP.,...&Lu, JX.(2014).Gold Nanoparticle-Based Enzyme-Linked Antibody-Aptamer Sandwich Assay for Detection of Salmonella Typhimurium.ACS APPLIED MATERIALS & INTERFACES,6(19),16974—16981. |
MLA | Wu, WH,et al."Gold Nanoparticle-Based Enzyme-Linked Antibody-Aptamer Sandwich Assay for Detection of Salmonella Typhimurium".ACS APPLIED MATERIALS & INTERFACES 6.19(2014):16974—16981. |
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