3D local structure around copper site of rabbit prion-related protein: Quantitative determination by XANES spectroscopy combined with multiple-scattering calculations

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	3D local structure around copper site of rabbit prion-related protein: Quantitative determination by XANES spectroscopy combined with multiple-scattering calculations
	Cui, PX ; Lian, FL ; Wang, Y ; Wen, Y ; Chu, WS ; Zhao, HF ; Zhang, S ; Li, J ; Lin, DH ; Wu, ZY
刊名	RADIATION PHYSICS AND CHEMISTRY
	2014
卷号	95 页码:69—72
关键词	X-RAY-ABSORPTION SPECTRA SCRAPIE
ISSN号	0969-806X
通讯作者	chuws@ustc.edu.cn ; dhlin@xmu.edu.cn ; wuzy@ustc.edu.cn
英文摘要	Prion-related protein (PrP), a cell-surface copper-binding glycoprotein, is considered to be responsible for a number of transmissible spongiform encephalopathies (TSEs). The structural conversion of PrP from the normal cellular isoform (PrPC) to the post-translationally modified form (PrPSc) is thought to be relevant to Cu2+ binding to histidine residues. Rabbits are one of the few mammalian species that appear to be resistant to TSEs, because of the structural characteristics of the rabbit prion protein (RaPrPC) itself. Here we determined the three-dimensional local structure around the C-terminal high-affinity copper-binding sites using X-ray absorption near-edge structure combined with ab initio calculations in the framework of the multiple-scattering (MS) theory. Result shows that two amino acid resides, Gln97 and Met108, and two histidine residues, His95 and His110, are involved in binding this copper(II) ion. It might help us understand the roles of copper in prion conformation conversions, and the molecular mechanisms of prion-involved diseases. (C) 2013 Elsevier Ltd. All rights reserved.
收录类别	SCI
语种	英语
WOS记录号	WOS:000329271400016
公开日期	2015-03-13
内容类型	期刊论文
源URL	[http://ir.sinap.ac.cn/handle/331007/13988]
专题	上海应用物理研究所_中科院上海应用物理研究所2011-2017年
推荐引用方式 GB/T 7714	Cui, PX,Lian, FL,Wang, Y,et al. 3D local structure around copper site of rabbit prion-related protein: Quantitative determination by XANES spectroscopy combined with multiple-scattering calculations[J]. RADIATION PHYSICS AND CHEMISTRY,2014,95:69—72.
APA	Cui, PX.,Lian, FL.,Wang, Y.,Wen, Y.,Chu, WS.,...&Wu, ZY.(2014).3D local structure around copper site of rabbit prion-related protein: Quantitative determination by XANES spectroscopy combined with multiple-scattering calculations.RADIATION PHYSICS AND CHEMISTRY,95,69—72.
MLA	Cui, PX,et al."3D local structure around copper site of rabbit prion-related protein: Quantitative determination by XANES spectroscopy combined with multiple-scattering calculations".RADIATION PHYSICS AND CHEMISTRY 95(2014):69—72.