Evaluation of the damage of cell wall and cell membrane for various extracellular polymeric substance extractions of activated sludge

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	Evaluation of the damage of cell wall and cell membrane for various extracellular polymeric substance extractions of activated sludge
	Xiao, Benyi
刊名	JOURNAL OF BIOTECHNOLOGY
	2014
卷号	188 期号:1 页码:130-135
关键词	Activated sludge Cell wall Cell membrane Damage Extracellular polymeric substances extraction
ISSN号	0168-1656
中文摘要	Extracellular polymeric substances (EPS) are susceptible to contamination by intracellular substances released during the extraction of EPS owing to the damage caused to microbial cell structures. The damage to cell walls and cell membranes in nine EPS extraction processes of activated sludge was evaluated in this study. The extraction of EPS (including proteins, carbohydrates and DNA) was the highest using the NaOH extraction method and the lowest using formaldehyde extraction. All nine EPS extraction methods in this study resulted in cell wall and membrane damage. The damage to cell walls, evaluated by 2-keto-3-deoxyoctonate (KDO) and N-acetylglucosamine content changes in extracted EPS, was the most significant in the NaOH extraction process. Formaldehyde extraction showed a similar extent of damage to cell walls to those detected in the control method (centrifugation), while those in the formaldehyde-NaOH and cation exchange resin extractions were slightly higher than those detected in the control. N-acetylglucosamine was more suitable than KDO for the evaluation of cell wall damage in the EPS extraction of activated sludge. The damage to cell membranes was characterized by two fluorochromes (propidium iodide and FITC Annexin V) with flow cytometry (FCM) measurement. The highest proportion of membrane-damaged cells was detected in NaOH extraction (26.54% of total cells) while membrane-damaged cells comprised 8.19% of total cells in the control. (C) 2014 Elsevier B.V. All rights reserved.
WOS记录号	WOS:000344183700019
公开日期	2015-03-26
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/9621]
专题	生态环境研究中心_水污染控制实验室
推荐引用方式 GB/T 7714	Xiao, Benyi. Evaluation of the damage of cell wall and cell membrane for various extracellular polymeric substance extractions of activated sludge[J]. JOURNAL OF BIOTECHNOLOGY,2014,188(1):130-135.
APA	Xiao, Benyi.(2014).Evaluation of the damage of cell wall and cell membrane for various extracellular polymeric substance extractions of activated sludge.JOURNAL OF BIOTECHNOLOGY,188(1),130-135.
MLA	Xiao, Benyi."Evaluation of the damage of cell wall and cell membrane for various extracellular polymeric substance extractions of activated sludge".JOURNAL OF BIOTECHNOLOGY 188.1(2014):130-135.