Automated and sensitive determination of four anabolic androgenic steroids in urine by online turbulent flow solid-phase extraction coupled with liquid chromatography-tandem mass spectrometry: A novel approach for clinical monitoring and doping control

CORC > 生态环境研究中心 > 中国科学院生态环境研究中心 > 环境化学与生态毒理学国家重点实验室

	Automated and sensitive determination of four anabolic androgenic steroids in urine by online turbulent flow solid-phase extraction coupled with liquid chromatography-tandem mass spectrometry: A novel approach for clinical monitoring and doping control
	Guo, Feng ; Shao, Jing ; Liu, Qian ; Shi, Jian-bo ; Jiang, Gui-bin
刊名	TALANTA
	2014
卷号	125 期号:1 页码:432-438
关键词	Online turboflow solid-phase extraction Anabolic androgenic steroids Urine Clinical monitoring Doping control
ISSN号	0039-9140
中文摘要	A novel method for automated and sensitive analysis of testosterone, androstenedione, methyltestosterone and methenolone in urine samples by online turbulent flow solid-phase extraction coupled with high performance liquid chromatography-tandem mass spectrometry was developed. The optimization and validation of the method were discussed in detail. The Turboflow C18-P SPE column showed the best extraction efficiency for all the analytes. Nanogram per liter (ng/L) level of MS could be determined directly and the limits of quantification (LOQs) were 0.01 ng/mL, which were much lower than normally concerned concentrations for these typical anabolic androgenic steroids (AAS) (0.1 ng/mL). The linearity range was from the LOQ to 100 ng/mL for each compound, with the coefficients of determination (r(2)) ranging from 0.9990 to 0.9999. The intraday and interday relative standard deviations (RSDs) ranged from 1.1% to 14.5% (n=5). The proposed method was successfully applied to the analysis of urine samples collected from 24 male athletes and 15 patients of prostate cancer. The proposed method provides an alternative practical way to rapidly determine MS in urine samples, especially for clinical monitoring and doping control. (C) 2014 Elsevier B.V. All rights reserved.
WOS记录号	WOS:000336872200062
公开日期	2015-03-24
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/9319]
专题	生态环境研究中心_环境化学与生态毒理学国家重点实验室
推荐引用方式 GB/T 7714	Guo, Feng,Shao, Jing,Liu, Qian,et al. Automated and sensitive determination of four anabolic androgenic steroids in urine by online turbulent flow solid-phase extraction coupled with liquid chromatography-tandem mass spectrometry: A novel approach for clinical monitoring and doping control[J]. TALANTA,2014,125(1):432-438.
APA	Guo, Feng,Shao, Jing,Liu, Qian,Shi, Jian-bo,&Jiang, Gui-bin.(2014).Automated and sensitive determination of four anabolic androgenic steroids in urine by online turbulent flow solid-phase extraction coupled with liquid chromatography-tandem mass spectrometry: A novel approach for clinical monitoring and doping control.TALANTA,125(1),432-438.
MLA	Guo, Feng,et al."Automated and sensitive determination of four anabolic androgenic steroids in urine by online turbulent flow solid-phase extraction coupled with liquid chromatography-tandem mass spectrometry: A novel approach for clinical monitoring and doping control".TALANTA 125.1(2014):432-438.