Rhizosphere effect has no effect on marker genes related to autotrophic CO2 fixation in paddy soils?

CORC > 生态环境研究中心 > 中国科学院生态环境研究中心 > 城市与区域生态国家重点实验室

	Rhizosphere effect has no effect on marker genes related to autotrophic CO2 fixation in paddy soils?
	Xiao, Ke-Qing ; Nie, San-An ; Bao, Peng ; Wang, Feng-Hua ; Bao, Qiong-Li ; Zhu, Yong-Guan
刊名	JOURNAL OF SOILS AND SEDIMENTS
	2014
卷号	14 期号:6 页码:1082-1087
关键词	CO2 fixation Marker genes Paddy soil Rhizosphere effect
ISSN号	1439-0108
中文摘要	Purpose The rhizosphere is a hotspot for microbial activities as well as microbial ecology studies. This study aimed to explore the "rhizosphere effect" on marker genes of CO2 fixation autotrophic microorganisms. Materials and methods Microcosm experiments were conducted using two different types of paddy soil, planted with rice and added with urea. At tillering and ripening stages, bulk and rhizosphere soils were sampled separately for DNA extraction and quantitative PCR analyses. Results and discussion Six marker genes (cbbLG, cbbLR, cbbM, aclB, oorA, accA) of three autotrophic pathways (the Calvin cycle, the reductive tricarboxylic acid cycle, and the 4-hydroxybutyrate cycle) were detected, suggesting their pervasiveness in paddy soil. Redundancy analysis and variation partitioning based on partial redundancy analysis revealed higher contributions of growth stage (explaining 37 %) and soil type (explaining 19 %) on the variation of these genes, lower but significant impact of rhizosphere effect (explaining 12 %), and no significant effect of urea addition (P>0.05). Conclusions These results suggested that the composition of CO2 fixation autotrophic microorganisms in the paddy soil was subject to combined actions of soil type and growth stage as well as rhizosphere effect.
WOS记录号	WOS:000336080800007
公开日期	2015-03-23
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/9224]
专题	生态环境研究中心_城市与区域生态国家重点实验室生态环境研究中心_土壤环境科学实验室
推荐引用方式 GB/T 7714	Xiao, Ke-Qing,Nie, San-An,Bao, Peng,et al. Rhizosphere effect has no effect on marker genes related to autotrophic CO2 fixation in paddy soils?[J]. JOURNAL OF SOILS AND SEDIMENTS,2014,14(6):1082-1087.
APA	Xiao, Ke-Qing,Nie, San-An,Bao, Peng,Wang, Feng-Hua,Bao, Qiong-Li,&Zhu, Yong-Guan.(2014).Rhizosphere effect has no effect on marker genes related to autotrophic CO2 fixation in paddy soils?.JOURNAL OF SOILS AND SEDIMENTS,14(6),1082-1087.
MLA	Xiao, Ke-Qing,et al."Rhizosphere effect has no effect on marker genes related to autotrophic CO2 fixation in paddy soils?".JOURNAL OF SOILS AND SEDIMENTS 14.6(2014):1082-1087.