Analysis of organogenic competence of cotyledons of Jatropha curcas and their in vitro histological behavior

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	Analysis of organogenic competence of cotyledons of Jatropha curcas and their in vitro histological behavior
	Nogueira, A. R. C.
刊名	African Journal of Biotechnology
	2011
卷号	10 期号:54 页码:11249-11258
ISSN号	1684-5315
中文摘要	Using cut pieces cotyledons from germinating zygotic embryos of Jatropha curcas, we monitored the series of anatomical events leading to the generation of shoot through organogenesis by histological analysis. 14 days old cotyledons that were pre-cultured in a half strength Murashige and Skoog (MS) media supplemented with 100 mg/L myoinositol and 10 mg/L thiamine HCl, were cultured in organogenic competence induction media (CIM) comprising of MS salts with 1.5 mg/L benzyl adenine (BA) and 0.05 mg/L indole-3-butyric acid (IBA) and incubated for the induction of organogenic competence. Following their sequential transfer to shoot induction media (containing MS + 1.5 mg/L BA, 0.05 mg/L IBA and 0.5 mg/L GA(3)); shoot elongation media (containing MS + 0.3 mg/L BA) and rooting media (containing half strength MS + IBA at different concentrations), we selected individual explants and subjected them to histological analysis in order to study the morphological changes occurring during organogenesis. Our findings show that to induce organogenesis using cut pieces of cotyledons, these tissues must be harvested at least by the 14th day following germination. Optimal organogenic competence was attained after 21 days incubation period in the dark where most of the explants showed evidence of protruding shoots surrounded by calli with various morphological features. Up to 53.91% of the total explants cultured in this study produced well defined shoots with each explant producing an average number of 1.5 shoots bearing two to eight leaves. We recorded the highest percentage of root formation, which stood at 27.50% when the shoots were cultured in a rooting media containing 0.3 mg/L IBA. Histological analysis of the different events occurring during the process of organogenesis suggest that the protuberances arising from parenchymatous cells and perhaps bundle sheath forming meristematic centres acquiring organogenic competence have a multicellular origin, indicating that the regeneration process takes place through direct organogenesis.
公开日期	2015-01-28
内容类型	期刊论文
源URL	[http://ir.xtbg.org.cn/handle/353005/7913]
专题	西双版纳热带植物园_热带植物资源可持续利用重点实验室特色文献_小桐子相关文献
推荐引用方式 GB/T 7714	Nogueira, A. R. C.. Analysis of organogenic competence of cotyledons of Jatropha curcas and their in vitro histological behavior[J]. African Journal of Biotechnology,2011,10(54):11249-11258.
APA	Nogueira, A. R. C..(2011).Analysis of organogenic competence of cotyledons of Jatropha curcas and their in vitro histological behavior.African Journal of Biotechnology,10(54),11249-11258.
MLA	Nogueira, A. R. C.."Analysis of organogenic competence of cotyledons of Jatropha curcas and their in vitro histological behavior".African Journal of Biotechnology 10.54(2011):11249-11258.