Adventitious rooting of Jatropha curcas L. is stimulated by phloroglucinol and by red LED light

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	Adventitious rooting of Jatropha curcas L. is stimulated by phloroglucinol and by red LED light
	Daud, N.
刊名	In Vitro Cellular & Developmental Biology-Plant
	2013
卷号	49 期号:2 页码:183-190
ISSN号	1054-5476
中文摘要	An efficient root induction system has been established for in vitro-regenerated Jatropha curcas L. shoots. Callus formation on shoots transferred to auxin containing medium was found to be a prominent and recurrent problem for rooting of in vitro-cultivated J. curcas. In particular, the type of auxins and cytokinins applied in the culture media were shown to strongly influence the severity of callus formation. Shoots cultivated on meta-methoxytopolin riboside (MemTR) were free of callus and produced elongated stems and well-developed leaves in comparison to the cytokinins benzyl adenine, zeatin, and thidiazuron. Subsequent root induction experiments were performed with shoots precultured on MemTR-containing medium. Shoots were excised and transferred to Murashige and Skoog (MS) medium supplemented with different concentrations of indole-3-butyric acid (IBA), indole-3-acetic acid (IAA), and alpha-naphtaleneacetic acid (NAA). The induction of excessive callus formation was avoided only on IBA-containing medium. The optimum rooting medium with good root induction (35%) and 1.2 roots per shoot contained half-strength MS salts supplemented with 2.5 mu M IBA. The same medium supplemented with 0.25% (w/v) activated charcoal produced 46% rooted shoots. Further improvement of rooting was obtained by transferring in vitro grown shoots to woody plant medium containing phloroglucinol (PG). In the presence of 2.5 mu M IBA and 238 mu M PG, 83% of the shoots rooted with on average 3.1 roots per shoot. We also analyzed the impact of light quality on the rooting capacity of Jatropha in vitro grown shoots. In general, light-emitting diodes (LEDs) light sources were less efficient for root induction. Red LED light provided the most favorable growth conditions, inducing a rooting response in 65% of the shoots, which produced on average 5.5 roots per shoot. These results indicate that adventitious rooting in J. curcas is under control of photoreceptors and that optimal rooting requires fine-tuning of the salt concentration, auxin, and cytokinin balance and application of synergistic compounds.
公开日期	2015-01-28
内容类型	期刊论文
源URL	[http://ir.xtbg.org.cn/handle/353005/7837]
专题	西双版纳热带植物园_热带植物资源可持续利用重点实验室特色文献_小桐子相关文献
推荐引用方式 GB/T 7714	Daud, N.. Adventitious rooting of Jatropha curcas L. is stimulated by phloroglucinol and by red LED light[J]. In Vitro Cellular & Developmental Biology-Plant,2013,49(2):183-190.
APA	Daud, N..(2013).Adventitious rooting of Jatropha curcas L. is stimulated by phloroglucinol and by red LED light.In Vitro Cellular & Developmental Biology-Plant,49(2),183-190.
MLA	Daud, N.."Adventitious rooting of Jatropha curcas L. is stimulated by phloroglucinol and by red LED light".In Vitro Cellular & Developmental Biology-Plant 49.2(2013):183-190.