A simplified method for extraction of high quality genomic DNA from Jatropha curcas for genetic diversity and molecular marker studies

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	A simplified method for extraction of high quality genomic DNA from Jatropha curcas for genetic diversity and molecular marker studies
	Pamidimarri, Dvns
刊名	Indian Journal of Biotechnology
	2009
卷号	8 期号:2 页码:187-192
ISSN号	0972-5849
中文摘要	A simple and efficient protocol for the extraction of high quality genomic DNA from different tissues, including callus generated from leaves of Jatropha curcas has been developed. The important steps in this protocol include (a) use of 3.5 M NaCl in extraction buffer; (b) 2.0 M NaCl (final concentration) during precipitation; (c) Tris saturated phenol in place of phenol: chloroform: isoamyl alcohol at purification phase; (d) 80% ethanol for DNA precipitation, and (e) performing all the steps at RT. The DNA thus extracted from the leaves had 1.81 +/- 0.063, OD at A(260/280) and the yield was 120 to 140 mu g/g of material. The extracted DNA was found suitable for restriction digestion, ligation and PCR amplification. It was also used for DNA fingerprinting techniques, RAPD and AFLP, for development of molecular markers and studies on genetic diversity.
公开日期	2015-01-28
内容类型	期刊论文
源URL	[http://ir.xtbg.org.cn/handle/353005/7673]
专题	西双版纳热带植物园_热带植物资源可持续利用重点实验室特色文献_小桐子相关文献
推荐引用方式 GB/T 7714	Pamidimarri, Dvns. A simplified method for extraction of high quality genomic DNA from Jatropha curcas for genetic diversity and molecular marker studies[J]. Indian Journal of Biotechnology,2009,8(2):187-192.
APA	Pamidimarri, Dvns.(2009).A simplified method for extraction of high quality genomic DNA from Jatropha curcas for genetic diversity and molecular marker studies.Indian Journal of Biotechnology,8(2),187-192.
MLA	Pamidimarri, Dvns."A simplified method for extraction of high quality genomic DNA from Jatropha curcas for genetic diversity and molecular marker studies".Indian Journal of Biotechnology 8.2(2009):187-192.