Development of an Ussuri catfish Pseudobagrus ussuriensis skin cell line displaying differential cytopathic effects to three aquatic animal viruses

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	Development of an Ussuri catfish Pseudobagrus ussuriensis skin cell line displaying differential cytopathic effects to three aquatic animal viruses
	Ou, Tong 1; Lei, Xiao-Ying 1; He, Li-Bo 1; Zhou, Feng-Jian 2; Zhang, Qi-Ya 1
刊名	VIRUS RESEARCH
	2014-08-30
卷号	189 页码:56-62
关键词	Ussuri catfish skin (UCS) cell line Cytopathic effect (CPE) Spring viremia of carp virus (SVCV) Grass carp reovirus (GCRV) Rana grylio virus (RGV) Recombinant iridovirus
ISSN号	0168-1702
英文摘要	An Ussuri catfish Pseudobagrus ussuriensis skin (UCS) cell line was developed and subcultured for more than 60 passages. UCS cells consisted of mostly epithelial-like cells and multiplied well in TC199 medium supplemented with 10% fetal bovine serum at 25 degrees C. Chromosome analysis revealed that most UCS cells had a normal diploid karyotype with 2n = 52. UCS cells showed differential cytopathic effects (CPEs) after inoculation of spring viremia of carp virus (SVCV, a negative-strand RNA virus), grass carp reovirus (GCRV, a multi-segmented double-stranded RNA virus) and Rana grylio virus (RGV, a large double-stranded DNA virus), and were indicative of high sensitivities to these three aquatic animal viruses by a virus titration study. The CPE caused by SVCV appeared as rounded and granular cells, grape-like clusters and small lytic plaques. Characteristic CPE containing plaque-like syncytia was induced by GCRV. RGV-infected cells produced typical CPE characterized by cells shrinkage and aggregation, formation of clear plaques and cell sheet detachment. Furthermore, significant fluorescent signals were observed after UCS cells were transfected with green fluorescent protein reporter plasmids, and the development of CPE induced by a recombinant RGV, Delta TK-RGV, in UCS cells was illustrated using a combination of light and fluorescence microscopy. The data from this study suggested that UCS cell line can potentially serve as a useful tool for the comparison study of different aquatic animal viruses and the isolation of some newly emerging viruses in Ussuri catfish farming. (C) 2014 Elsevier B.V. All rights reserved.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Virology
研究领域[WOS]	Virology
关键词[WOS]	RANA-GRYLIO VIRUS ; COMPLETE GENOME SEQUENCE ; RED-SEA BREAM ; CYPRINUS-CARPIO ; SPRING VIREMIA ; TELEOST FISH ; B-CELLS ; RHABDOVIRUS ; ESTABLISHMENT ; REOVIRUS
收录类别	SCI
语种	英语
WOS记录号	WOS:000341557700009
公开日期	2015-01-20
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/20344]
专题	水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文
作者单位	1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China 2.Huaian Fisheries Sci Res Inst Jiangsu Prov, Huaian 223001, Peoples R China
推荐引用方式 GB/T 7714	Ou, Tong,Lei, Xiao-Ying,He, Li-Bo,et al. Development of an Ussuri catfish Pseudobagrus ussuriensis skin cell line displaying differential cytopathic effects to three aquatic animal viruses[J]. VIRUS RESEARCH,2014,189:56-62.
APA	Ou, Tong,Lei, Xiao-Ying,He, Li-Bo,Zhou, Feng-Jian,&Zhang, Qi-Ya.(2014).Development of an Ussuri catfish Pseudobagrus ussuriensis skin cell line displaying differential cytopathic effects to three aquatic animal viruses.VIRUS RESEARCH,189,56-62.
MLA	Ou, Tong,et al."Development of an Ussuri catfish Pseudobagrus ussuriensis skin cell line displaying differential cytopathic effects to three aquatic animal viruses".VIRUS RESEARCH 189(2014):56-62.