Dietary tryptophan modulates intestinal immune response, barrier function, antioxidant status and gene expression of TOR and Nrf2 in young grass carp (Ctenopharyngodon idella)

CORC > 水生生物研究所 > 中科院水生所知识产出（2009年前） > 水生生物分子与细胞生物学研究中心 > 期刊论文

	Dietary tryptophan modulates intestinal immune response, barrier function, antioxidant status and gene expression of TOR and Nrf2 in young grass carp (Ctenopharyngodon idella)
	Wen, Hailang 1; Feng, Lin 1,2,3; Jiang, Weidan 1,2,3; Liu, Yang 1,2,3; Jiang, Jun 1,2,3; Li, Shuhong 1; Tang, Ling 4; Zhang, Yongan 5; Kuang, Shengyao 4; Zhou, Xiaoqiu 1,2,3
刊名	FISH & SHELLFISH IMMUNOLOGY
	2014-09-01
卷号	40 期号:1 页码:275-287
关键词	Tryptophan Immune response Tight junction protein Antioxidant status Nrf2
ISSN号	1050-4648
英文摘要	The present research evaluated the effects of dietary tryptophan (Trp) on growth performance, intestinal mucosa] immune, barrier function and antioxidant capacity and gene expression of young grass carp (Ctenophatyngodon idella). Fish were fed six different experimental diets containing graded levels of Trp at 0.7(control), 1.7, 3.1, 4.0, 52 and 6.1 g kg(-1) diet for 8 weeks. The results showed that Trp supplementation significantly enhanced the percent weight gain (PWG), feed intake and feed efficiency (P < 0.05), and decreased the plasma ammonia content (PAC) (P < 0.05). After the 8-week feeding trail, an environmental copper exposure trail was conducted for 4 days. Results from the copper exposure trail showed that dietary Trp enhanced the lysozyme, acid phosphatase activities and complement 3 contents in the intestine of young grass carp (P < 0.05). In addition, Trp supplementation increased the copper/zinc superoxide dismutase (SOD1), glutathione peroxidase (GPx) activities and glutathione contents (P < 0.05), and decreased the protein carbonyl and malondialdehyde contents (P < 0.05). Furthermore, the relative gene expression levels of interleukin 10, transforming growth factor-beta 1, occludin, zonula occludens 1, claudin-b, -c, and 3, SOD1, GPx and NF-E2-related factor 2 in the intestine were significantly up-regulated with increasing of dietary Trp up to a certain level (P < 0.05). Conversely, the mRNA levels of tumor necrosis factor a, interleukin 8, target of rapamycin, Kelch-like-ECH-associated protein 1, claudin-12 and -15a in the intestine were significantly down-regulated by Trp (P < 0.05). Collectively, appropriate dietary Trp level improves fish growth, intestinal immune response, barrier function and antioxidant status, and regulated the mRNA levels of related signal molecules of young grass carp. Based on the quadratic regression analysis of the PWG and PAC, the dietary Trp requirement of young grass carp (287-699 g) was estimated to be 3.81 g kg(-1) diet (12.7 g kg(-1) protein) and 3.89 g kg(-1) diet (13.0 g kg(-1) protein), respectively. (C) 2014 Elsevier Ltd. All rights reserved.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
研究领域[WOS]	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
关键词[WOS]	SALMON SALMO-SALAR ; TROUT ONCORHYNCHUS-MYKISS ; INDUCED OXIDATIVE DAMAGE ; JUVENILE JIAN ; VAR. JIAN ; ENZYME-ACTIVITIES ; GROWTH-PERFORMANCE ; BODY-COMPOSITION ; PLASMA-CORTISOL ; MESSENGER-RNA
收录类别	SCI
语种	英语
WOS记录号	WOS:000341468500033
公开日期	2015-01-20
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/20384]
专题	水生生物研究所_水生生物分子与细胞生物学研究中心_期刊论文
作者单位	1.Sichuan Agr Univ, Inst Anim Nutr, Chengdu 611130, Sichuan, Peoples R China 2.Sichuan Agr Univ, Fish Nutr & Safety Prod Univ Key Lab Sichuan Prov, Chengdu 611130, Sichuan, Peoples R China 3.Sichuan Agr Univ, China Minist Educ, Key Lab Anim Dis Resistance Nutr, Chengdu 611130, Sichuan, Peoples R China 4.Sichuan Acad Anim Sci, Inst Anim Nutr, Chengdu 610066, Peoples R China 5.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
推荐引用方式 GB/T 7714	Wen, Hailang,Feng, Lin,Jiang, Weidan,et al. Dietary tryptophan modulates intestinal immune response, barrier function, antioxidant status and gene expression of TOR and Nrf2 in young grass carp (Ctenopharyngodon idella)[J]. FISH & SHELLFISH IMMUNOLOGY,2014,40(1):275-287.
APA	Wen, Hailang.,Feng, Lin.,Jiang, Weidan.,Liu, Yang.,Jiang, Jun.,...&Zhou, Xiaoqiu.(2014).Dietary tryptophan modulates intestinal immune response, barrier function, antioxidant status and gene expression of TOR and Nrf2 in young grass carp (Ctenopharyngodon idella).FISH & SHELLFISH IMMUNOLOGY,40(1),275-287.
MLA	Wen, Hailang,et al."Dietary tryptophan modulates intestinal immune response, barrier function, antioxidant status and gene expression of TOR and Nrf2 in young grass carp (Ctenopharyngodon idella)".FISH & SHELLFISH IMMUNOLOGY 40.1(2014):275-287.