Analysis of phosphoproteome in rice pistil

CORC > 水生生物研究所 > 中科院水生所知识产出（2009年前） > 水生生物分子与细胞生物学研究中心 > 期刊论文

	Analysis of phosphoproteome in rice pistil
	Wang, Kun 1; Zhao, Yong 2; Li, Ming 1; Gao, Feng 3; Yang, Ming-kun 4; Wang, Xin 1; Li, Shaoqing 3; Yang, Pingfang 1
刊名	PROTEOMICS
	2014-10-01
卷号	14 期号:20 页码:2319-2334
关键词	IMAC Oryza sativa L. Phosphoproteome Pistil Plant proteomics Pollen-pistil interaction
ISSN号	1615-9853
英文摘要	As the female reproductive part of a flower, the pistil consists of the ovary, style, and stigma, and is a critical organ for the process from pollen recognition to fertilization and seed formation. Previous studies on pollen-pistil interaction mainly focused on gene expression changes with comparative transcriptomics or proteomics method. However, studies on protein PTMs are still lacking. Here we report a phosphoproteomic study on mature pistil of rice. Using IMAC enrichment, hydrophilic interaction chromatography fraction and high-accuracy MS instrument (TripleTOF 5600), 2347 of high-confidence (Ascore >= 19, p <= 0.01), phosphorylation sites corresponding to 1588 phosphoproteins were identified. Among them, 1369 phosphorylation sites within 654 phosphoproteins were newly identified; 41 serine phosphorylation motifs, which belong to three groups: proline-directed, basophilic, and acidic motifs were identified after analysis by motif-X. Two hundred and one genes whose phosphopeptides were identified here showed tissue-specific expression in pistil based on information mining of previous microarray data. All MS data have been deposited in the ProteomeXchange with identifier PXD000923 (http://proteomecentral.proteomexchange.org/dataset/PXD000923). This study will help us to understand pistil development and pollination on the posttranslational level.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biochemical Research Methods ; Biochemistry & Molecular Biology
研究领域[WOS]	Biochemistry & Molecular Biology
关键词[WOS]	SELF-INCOMPATIBILITY RESPONSE ; LOCUS RECEPTOR KINASE ; POLLEN-TUBE GROWTH ; PROTEIN-PHOSPHORYLATION ANALYSIS ; GENE-EXPRESSION ; S-LOCUS ; ARABIDOPSIS POLLEN ; ORYZA-SATIVA ; PROTEOMIC ANALYSIS ; PROFILING REVEALS
收录类别	SCI
语种	英语
WOS记录号	WOS:000343833700010
公开日期	2015-01-20
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/20378]
专题	水生生物研究所_水生生物分子与细胞生物学研究中心_期刊论文
作者单位	1.Chinese Acad Sci, Wuhan Bot Garden, Key Lab Plant Germplasm Enhancement & Specialty A, Wuhan 430074, Peoples R China 2.Wuhan Univ, Coll Life Sci, Wuhan 430072, Peoples R China 3.Wuhan Univ, Coll Life Sci, State Key Lab Hybrid Rice, Wuhan 430072, Peoples R China 4.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan, Peoples R China
推荐引用方式 GB/T 7714	Wang, Kun,Zhao, Yong,Li, Ming,et al. Analysis of phosphoproteome in rice pistil[J]. PROTEOMICS,2014,14(20):2319-2334.
APA	Wang, Kun.,Zhao, Yong.,Li, Ming.,Gao, Feng.,Yang, Ming-kun.,...&Yang, Pingfang.(2014).Analysis of phosphoproteome in rice pistil.PROTEOMICS,14(20),2319-2334.
MLA	Wang, Kun,et al."Analysis of phosphoproteome in rice pistil".PROTEOMICS 14.20(2014):2319-2334.