Allograft inflammatory factor-1 stimulates hemocyte immune activation by enhancing phagocytosis and expression of inflammatory cytokines in Crassostrea gigas

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	Allograft inflammatory factor-1 stimulates hemocyte immune activation by enhancing phagocytosis and expression of inflammatory cytokines in Crassostrea gigas
	[Zhang, Yang ; Li, Jun ; Yu, Feng ; He, Xiaocui ; Yu, Ziniu] Chinese Acad Sci, South China Sea Inst Oceanol, Lab Appl Marine Biol, Key Lab Marine Bioresources Sustainable Utilizat, Guangzhou 510301, Guangdong, Peoples R China
刊名	FISH & SHELLFISH IMMUNOLOGY
	2013
卷号	34 期号:5 页码:1071-1077
关键词	Crassostrea gigas AIF-1 Hemocyte activation Phagocytosis Inflammatory cytokine
ISSN号	1050-4648
通讯作者	carlzyu@scsio.ac.cn
中文摘要	Allograft inflammatory factor-1 (AIF-1) is a calcium-binding cytokine associated with immune cell activation and inflammatory response. Presently, we have identified and characterized an AIF-1 in a marine bivalve mollusk, Crassostrea gigas, and designated it as CgAIF-1. The full-length CgAIF-1 cDNA is 794 bp, encoding a protein of 149 amino acids with two conserved EF hand Ca2+-binding motifs. CgAIF-1 is constitutively expressed in various tissues with enriched expression in hemocytes. Moreover, CgAIF-1 transcription is induced by multiple Pathogen-Associated Molecular Patterns (PAMPs), including poly (I: C), LPS, PGN, HKLM and HKVA, but is limited by 1,3-beta-glucan. Furthermore, recombinant CgAIF-1 can specifically stimulate phagocytic ability of granulocytes, but not of intermediate cells and hyalinocytes. CgAIF-1 also enhances mRNA levels of MIF, TNF and IL-17. These results provide the first functional evidence that CgAIF-1 is involved in hemocyte activation in C gigas, revealing conserved functions of AIF-1 in host defense from mollusks to mammals. (C) 2013 Elsevier Ltd. All rights reserved.
学科主题	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
资助信息	This work was supported by the National Science Foundation of China (No. 31001130 and 41176150), the National Basic Research Program of China (No. 2010CB126404) and the Joint Funds of NSFC-Guangdong of China (U1201215). We also thank Prof. Elizabeth De Stasio for critical reading and editing of the manuscript.
原文出处	ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
语种	英语
WOS记录号	WOS:000317870600007
公开日期	2015-01-22
内容类型	期刊论文
源URL	[http://ir.scsio.ac.cn/handle/344004/11038]
专题	南海海洋研究所_中科院海洋生物资源可持续利用重点实验室
推荐引用方式 GB/T 7714	[Zhang, Yang,Li, Jun,Yu, Feng,et al. Allograft inflammatory factor-1 stimulates hemocyte immune activation by enhancing phagocytosis and expression of inflammatory cytokines in Crassostrea gigas[J]. FISH & SHELLFISH IMMUNOLOGY,2013,34(5):1071-1077.
APA	[Zhang, Yang,Li, Jun,Yu, Feng,He, Xiaocui,&Yu, Ziniu] Chinese Acad Sci, South China Sea Inst Oceanol, Lab Appl Marine Biol, Key Lab Marine Bioresources Sustainable Utilizat, Guangzhou 510301, Guangdong, Peoples R China.(2013).Allograft inflammatory factor-1 stimulates hemocyte immune activation by enhancing phagocytosis and expression of inflammatory cytokines in Crassostrea gigas.FISH & SHELLFISH IMMUNOLOGY,34(5),1071-1077.
MLA	[Zhang, Yang,et al."Allograft inflammatory factor-1 stimulates hemocyte immune activation by enhancing phagocytosis and expression of inflammatory cytokines in Crassostrea gigas".FISH & SHELLFISH IMMUNOLOGY 34.5(2013):1071-1077.