Identification and characterization of TRP14, a thioredoxin-related protein of 14 kDa from orange-spotted grouper, Epinephelus coioides

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	Identification and characterization of TRP14, a thioredoxin-related protein of 14 kDa from orange-spotted grouper, Epinephelus coioides
	[Wei, Jingguang ; Guo, Minglan ; Yan, Yang ; Qin, Qiwei] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China ; [Ji, Huasong] Hainan Univ, Coll Marine Sci, State Key Lab Breeding Base Sustainable Exploitat, Haikou 570228, Peoples R China
刊名	FISH & SHELLFISH IMMUNOLOGY
	2013
卷号	35 期号:5 页码:1670-1676
关键词	Epinephelus coioides Singapore grouper iridovirus (SGIV) Thioredoxin-related protein of 14 kDa (Ec-TRP14) Antioxidant activity
ISSN号	1050-4648
通讯作者	qinqw@scsio.ac.cn
中文摘要	Thioredoxin (abbreviated as Trx) is an important ubiquitous disulfide reductase, which can protect organisms against various oxidative stresses. In the present study, a thioredoxin-related protein of 14 kDa (named as Ec-TRP14) was identified from the marine fish grouper, Epinephelus coloides by RACE PCR. The full-length cDNA of Ec-TRP14 was comprised of 1066 bp with a 372 bp open reading frame that encodes a putative protein of 123 amino acids. Similar to most TRP145, Ec-TRP14 contained the conserved motif C-P-D-C. Ec-TRP14 mRNA is predominately expressed in liver, brain and muscle. The expression of Ec-TRP14 was up-regulated in the liver of grouper challenged with SGIV. Ec-TRP14 was recombined and expressed in Escherichia call BL21 (DE3), and the rEc-Ec-TRP14 fusion protein was demonstrated to possess the antioxidant activity. The grouper spleen (GS) cells were treated with a high concentration of rEc-TRP14 (8.3 mu g/ml), which significantly enhanced cells viability under damage caused by viral infection. These results together indicated that Ec-TRP14 could function as an important antioxidant in a physiological context, and might be involved in the responses to viral challenge. (C) 2013 Elsevier Ltd. All rights reserved.
学科主题	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
资助信息	This work was supported by grants from National High Technology Development Program of China (863) (2012AA092201), National Basic Research Program of China (973 program) (2012CB114402), the Knowledge Innovation Program of the Chinese Academy of Sciences (KZCX2-EW-Q213, KZCX2-YW-BR-08, SQ201203), the National Natural Science Foundation of China (30930070, 31001124, 30725027, 31202022), and the Guangdong Province Natural Science Foundation (S2011040000953, 2012A020603018).
原文出处	ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
语种	英语
WOS记录号	WOS:000328006700038
公开日期	2015-01-22
内容类型	期刊论文
源URL	[http://ir.scsio.ac.cn/handle/344004/10968]
专题	南海海洋研究所_中科院海洋生物资源可持续利用重点实验室
推荐引用方式 GB/T 7714	[Wei, Jingguang,Guo, Minglan,Yan, Yang,et al. Identification and characterization of TRP14, a thioredoxin-related protein of 14 kDa from orange-spotted grouper, Epinephelus coioides[J]. FISH & SHELLFISH IMMUNOLOGY,2013,35(5):1670-1676.
APA	[Wei, Jingguang,Guo, Minglan,Yan, Yang,Qin, Qiwei] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China,&[Ji, Huasong] Hainan Univ, Coll Marine Sci, State Key Lab Breeding Base Sustainable Exploitat, Haikou 570228, Peoples R China.(2013).Identification and characterization of TRP14, a thioredoxin-related protein of 14 kDa from orange-spotted grouper, Epinephelus coioides.FISH & SHELLFISH IMMUNOLOGY,35(5),1670-1676.
MLA	[Wei, Jingguang,et al."Identification and characterization of TRP14, a thioredoxin-related protein of 14 kDa from orange-spotted grouper, Epinephelus coioides".FISH & SHELLFISH IMMUNOLOGY 35.5(2013):1670-1676.