Characterization of an envelope gene VP19 from Singapore grouper iridovirus

CORC > 南海海洋研究所 > 中国科学院南海海洋研究所 > 中科院海洋生物资源可持续利用重点实验室

	Characterization of an envelope gene VP19 from Singapore grouper iridovirus
	[Huang, Xiaohong ; Huang, Youhua ; Ouyang, Zhengliang ; Wang, Shaowen ; Chen, Xiuli ; Qin, Qiwei] Chinese Acad Sci, South China Sea Inst Oceanol, Key Lab Trop Marine Bioresources & Ecol, Guangzhou 510301, Guangdong, Peoples R China ; [Gong, Jie] Sun Yat Sen Univ, Sch Life Sci, State Key Lab Biocontrol, Guangzhou 510275, Guangdong, Peoples R China
刊名	VIROLOGY JOURNAL
	2013
卷号	10 页码:-354
关键词	SGIV Iridovirus VP19 Envelope protein Virus assembly
ISSN号	1743-422X
其他题名	mannual of IR1
通讯作者	qinqw@scsio.ac.cn
产权排序	产权排序
合作状况	填入合作性质等
中文摘要	Background: Viral envelope proteins are always proposed to exert important function during virus infection and replication. Vertebrate iridoviruses are enveloped large DNA virus, which can cause great economic losses in aquaculture and ecological destruction. Although numerous iridovirus envelope proteins have been identified using bioinformatics and proteomic methods, their roles in virus infection remained largely unknown. Methods: Using SMART and TMHMM programs, we investigated the structural characteristics of Singapore grouper iridovirus (SGIV) VP19. A specific antibody against VP19 was generated and the expression profile of VP19 was clarified. The subcellular localization of VP19 in the absence or presence of other viral products was determined via transfection and immune fluorescence assay. In addition, Western blot assay and electron microscopy examination were performed to demonstrate whether SGIV VP19 was an envelope protein or a capsid protein. Results: Here, SGIV VP19 was cloned and characterized. Among all sequenced iridoviruses, VP19 and its orthologues shared common features, including 19 invariant cysteines, a proline-rich motif and a predicted transmembrane domain. Subsequently, the protein synthesis of VP19 was only detected at the late stage of SGIV infection and inhibited obviously by treating with AraC, confirming that VP19 was a late expressed protein. Ectopic expression of EGFP-VP19 in vitro displayed a punctate pattern in the cytoplasm. In SGIV infected cells, the newly synthesized VP19 protein was initially localized in the cytoplasm in a punctate pattern, and then aggregated into the virus assembly site at the late stage of SGIV infection, suggesting that other viral protein products were essential for VP19's function during SGIV infection. In addition, Western blot assay and electron microscopy observation revealed that SGIV VP19 was associated with viral envelope, which was different from major capsid protein (MCP). Conclusion: Taken together, the current data suggested that VP19 represented a conserved envelope protein in iridovirus, and might contribute greatly to virus assembly during virus infection.
英文摘要	description.abstract
学科主题	Virology
收录类别	SCI
资助信息	This work was supported by grants from the National Basic Research Program of China (973) (2012CB114402, 2012CB114406), the National Natural Science Foundation of China (31172445, 30930070, 31172437), the Knowledge Innovation Program of the Chinese Academy of Sciences (KSCX2-EW-G-12B) and the National High-Tech Research and. Development Program (863) (SS2014AA091607).
原文出处	BIOMED CENTRAL LTD
语种	英语
WOS记录号	WOS:000330051600001
公开日期	2015-01-22
内容类型	期刊论文
源URL	[http://ir.scsio.ac.cn/handle/344004/10945]
专题	南海海洋研究所_中科院海洋生物资源可持续利用重点实验室
推荐引用方式 GB/T 7714	[Huang, Xiaohong,Huang, Youhua,Ouyang, Zhengliang,et al. Characterization of an envelope gene VP19 from Singapore grouper iridovirus[J]. VIROLOGY JOURNAL,2013,10:-354.
APA	[Huang, Xiaohong.,Huang, Youhua.,Ouyang, Zhengliang.,Wang, Shaowen.,Chen, Xiuli.,...&[Gong, Jie] Sun Yat Sen Univ, Sch Life Sci, State Key Lab Biocontrol, Guangzhou 510275, Guangdong, Peoples R China.(2013).Characterization of an envelope gene VP19 from Singapore grouper iridovirus.VIROLOGY JOURNAL,10,-354.
MLA	[Huang, Xiaohong,et al."Characterization of an envelope gene VP19 from Singapore grouper iridovirus".VIROLOGY JOURNAL 10(2013):-354.