Interleukin-1 beta guides the migration of cortical neurons

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	Interleukin-1 beta guides the migration of cortical neurons
	Ma, L ; Li, XW ; Zhang, SJ ; Yang, F ; Zhu, GM ; Yuan, XB ; Jiang, W
刊名	JOURNAL OF NEUROINFLAMMATION
	2014
卷号	11 期号:1 页码:114-114
关键词	NEURAL PRECURSOR CELLS RADIAL MIGRATION SUBVENTRICULAR ZONE TNF-ALPHA MALFORMATIONS PATHWAY GROWTH BRAIN RAT NEUROGENESIS
ISSN号	1742-2094
通讯作者	Jiang, W (reprint author), Fourth Mil Med Univ, Xijing Hosp, Dept Neurol, Xian 710032, Peoples R China.,jiangwen@fmmu.edu.cn
英文摘要	Background: Proinflammatory cytokine interleukin-1beta (IL-1 beta) is expressed at high levels in the developing brain and declines to low constitutive levels in the adult. However, the pathophysiological function of IL-1 beta during brain development remains elusive. In this study, we investigated the role of IL-1 beta in neuronal migration. Methods: The Boyden transwell assay was used to examine the effects of IL-1 beta on the migration of dissociated primary cortical neurons. To determine the role of IL-1 beta in neuron leading process pathfinding, we employed a growth cone turning assay. In utero electroporation combined with RNAi technology was used to examine the neuronal migration in vivo during brain development in Sprague-Dawley rats. Results: IL-1 beta at concentrations ranging from 0.1 to 10 ng/mL in the lower chamber of a transwell induced a significant increase in the number of migrating neurons in a dose-dependent manner. When IL-1 beta was simultaneously put in both the upper and lower chambers to eliminate the gradient, no significant differences in cell migration were observed. IL-1 receptor antagonist IL-1RA dose-dependently blocked the attractive effect of IL-1 on neuronal migration. Microscopic gradients of IL-1 beta were created near the growth cones of isolated neurons by repetitive pulsatile application of picoliters of a IL-1 beta-containing solution with a micropipette. We found that growth cones exhibited a clear bias toward the source of IL-1 beta at the end of a one hour period in the IL-1 beta gradient. No significant difference was observed in the rate of neurite extension between IL-1 beta and controls. We electroporated specific siRNA constructs against IL-1R1 mRNA into cortical progenitors at embryonic day 16 and examined the position and distribution of transfected cells in the somatosensory cortex at postnatal day 5. We found that neurons transfected with IL-1R1-siRNA displayed a severe retardation in radial migration, with about 83% of total cells unable to arrive at the upper cortical layers. Conclusions: Our study suggests an essential contribution of IL-1 beta to neuronal migration during brain development, which provides a basis to understand the physiological roles of IL-1 beta in the developing brain and could have significant implications for the prevention of some neurodevelopment disorders due to abnormal neuronal migration.
学科主题	Immunology ; Neurosciences
收录类别	SCI
资助信息	Natural Science Foundation of China [30870840, 81071051]; Program for Changjiang Scholars and Innovative Research Team in University from the Ministry of Education of China [IRT1053]
语种	英语
公开日期	2014-12-15
内容类型	期刊论文
源URL	[http://ir.sibs.ac.cn/handle/331001/2658]
专题	上海神经科学研究所_神经所(总)
推荐引用方式 GB/T 7714	Ma, L,Li, XW,Zhang, SJ,et al. Interleukin-1 beta guides the migration of cortical neurons[J]. JOURNAL OF NEUROINFLAMMATION,2014,11(1):114-114.
APA	Ma, L.,Li, XW.,Zhang, SJ.,Yang, F.,Zhu, GM.,...&Jiang, W.(2014).Interleukin-1 beta guides the migration of cortical neurons.JOURNAL OF NEUROINFLAMMATION,11(1),114-114.
MLA	Ma, L,et al."Interleukin-1 beta guides the migration of cortical neurons".JOURNAL OF NEUROINFLAMMATION 11.1(2014):114-114.