Directed evolution of cytochrome P450 for sterol epoxidation

CORC > 天津工业生物技术研究所 > 进化代谢工程和分子进化研究组王钦宏 > 期刊论文

	Directed evolution of cytochrome P450 for sterol epoxidation
	Jiang, Dan 1,2; Tu, Ran 2; Bai, Peng 1; Wang, Qinhong 2
刊名	BIOTECHNOLOGY LETTERS
	2013-10-01
卷号	35 期号:10 页码:1663-1668
关键词	Cytochrome P450 Directed evolution Epoxidation Sterol
英文摘要	16,17-Epoxysterol plays an important role in pharmaceutical steroid synthesis. To investigate the potential application of cytochrome P450 for epoxysterol synthesis, an approach to the epoxidation of 16,17-epoxysterol, based on directed evolution of cytochrome P450 BM-3, was developed. This comprised random gene mutagenesis for optimizing the activity of P450 BM-3 for epoxidation of hydrophobic sterol, followed by the 7-ethoxycoumarin de-ethylation assay for general enzyme activity detection and the modified picric acid assay for epoxidation activity screening. By the two-step screening, one mutant from 792 clones showed specific substrate activity of converting progesterone to 16,17-epoxysterol, which validated the possibility to evolve the cytochrome P450 for the synthesis of steroidal epoxides.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biotechnology & Applied Microbiology
研究领域[WOS]	Biotechnology & Applied Microbiology
关键词[WOS]	CHIRAL EPOXIDES ; BIOCATALYSIS ; P450BM-3
收录类别	SCI
语种	英语
WOS记录号	WOS:000325011900016
公开日期	2014-11-23
内容类型	期刊论文
源URL	[http://124.16.173.210/handle/311007/409]
专题	天津工业生物技术研究所_进化代谢工程和分子进化研究组王钦宏_期刊论文
作者单位	1.Tianjin Univ, Sch Chem Engn & Technol, Tianjin 300072, Peoples R China 2.Chinese Acad Sci, Key Lab Syst Microbial Biotechnol, Tianjin Inst Ind Biotechnol, Tianjin Airport Econ Area, Tianjin 300308, Peoples R China
推荐引用方式 GB/T 7714	Jiang, Dan,Tu, Ran,Bai, Peng,et al. Directed evolution of cytochrome P450 for sterol epoxidation[J]. BIOTECHNOLOGY LETTERS,2013,35(10):1663-1668.
APA	Jiang, Dan,Tu, Ran,Bai, Peng,&Wang, Qinhong.(2013).Directed evolution of cytochrome P450 for sterol epoxidation.BIOTECHNOLOGY LETTERS,35(10),1663-1668.
MLA	Jiang, Dan,et al."Directed evolution of cytochrome P450 for sterol epoxidation".BIOTECHNOLOGY LETTERS 35.10(2013):1663-1668.