A Replicating Modified Vaccinia Tiantan Strain Expressing an Avian-Derived Influenza H5N1 Hemagglutinin Induce Broadly Neutralizing Antibodies and Cross-Clade Protective Immunity in Mice

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	A Replicating Modified Vaccinia Tiantan Strain Expressing an Avian-Derived Influenza H5N1 Hemagglutinin Induce Broadly Neutralizing Antibodies and Cross-Clade Protective Immunity in Mice
	Xiao, Haixia 1,3; Liu, Li 2,4,5; Zhu, Qingyu 6; Tan, Zhiwu 2; Yu, Wenbo 2; Tang, Xian 2; Zhan, Dawei 6; Du, Yanhua 2; Wang, Haibo 2; Liu, Di 3
刊名	PLOS ONE
	2013-12-17
卷号	8 期号:12
英文摘要	To combat the possibility of a zoonotic H5N1 pandemic in a timely fashion, it is necessary to develop a vaccine that would confer protection against homologous and heterologous human H5N1 influenza viruses. Using a replicating modified vaccinia virus Tian Tan strain (MVTT) as a vaccine vector, we constructed MVTTHA-QH and MVTTHA-AH, which expresses the H5 gene of a goose-derived Qinghai strain A/Bar-headed Goose/Qinghai/1/2005 or human-derived Anhui Strain A/Anhui/1/2005. The immunogenicity profiles of both vaccine candidates were evaluated. Vaccination with MVTTHA-QH induced a significant level of neutralizing antibodies (Nabs) against a homologous strain and a wide range of H5N1 pseudoviruses (clades 1, 2.1, 2.2, 2.3.2, and 2.3.4). Neutralization tests (NT) and Haemagglutination inhibition (HI) antibodies inhibit the live autologous virus as well as a homologous A/Xingjiang/1/2006 and a heterologous A/Vietnam/1194/2004, representing two human isolates from clade 2.2 and clade 1, respectively. Importantly, mice vaccinated with intranasal MVTTHA-QH were completely protected from challenge with lethal dosages of A/Bar-headed Goose/Qinghai/1/2005 and the A/Viet Nam/1194/2004, respectively, but not control mice that received a mock MVTTS vaccine. However, MVTTHA-AH induced much lower levels of NT against its autologous strain. Our results suggest that it is feasible to use the H5 gene from A/Bar-headed Goose/Qinghai/1/2005 to construct an effective vaccine, when using MVTT as a vector, to prevent infections against homologous and genetically divergent human H5N1 influenza viruses.
WOS标题词	Science & Technology
类目[WOS]	Multidisciplinary Sciences
研究领域[WOS]	Science & Technology - Other Topics
关键词[WOS]	VIRUS ANKARA ; A H5N1 ; PANDEMIC INFLUENZA ; RECOMBINANT VECTOR ; RHESUS-MONKEYS ; TAN STRAIN ; HOST-RANGE ; HONG-KONG ; VACCINATION ; IMMUNOGENICITY
收录类别	SCI
语种	英语
WOS记录号	WOS:000328737700055
公开日期	2014-11-28
内容类型	期刊论文
源URL	[http://124.16.173.210/handle/311007/526]
专题	天津工业生物技术研究所_蛋白质工程与疫苗实验室高福_期刊论文
作者单位	1.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Lab Prot Engn & Vaccines, Tianjin, Peoples R China 2.Univ Hong Kong, Li Ka Shing Fac Med, AIDS Inst, Hong Kong, Hong Kong, Peoples R China 3.Chinese Acad Sci, Inst Microbiol, Key Lab Pathogen Microbiol & Immunol, Beijing, Peoples R China 4.Univ Hong Kong, Li Ka Shing Fac Med, Dept Microbiol, Hong Kong, Hong Kong, Peoples R China 5.Univ Hong Kong, Li Ka Shing Fac Med, Res Ctr Infect & Immunol, Hong Kong, Hong Kong, Peoples R China 6.Acad Mil Med Sci, Inst Microbiol & Epidemiol, State Key Lab Pathogens & Biosecur, Beijing, Peoples R China 7.Acad Sin, Genom Res Ctr, Taipei, Taiwan 8.Rockefeller Univ, Aaron Diamond AIDS Res Ctr, New York, NY USA 9.Univ Hong Kong, Hong Kong, Hong Kong, Peoples R China
推荐引用方式 GB/T 7714	Xiao, Haixia,Liu, Li,Zhu, Qingyu,et al. A Replicating Modified Vaccinia Tiantan Strain Expressing an Avian-Derived Influenza H5N1 Hemagglutinin Induce Broadly Neutralizing Antibodies and Cross-Clade Protective Immunity in Mice[J]. PLOS ONE,2013,8(12).
APA	Xiao, Haixia.,Liu, Li.,Zhu, Qingyu.,Tan, Zhiwu.,Yu, Wenbo.,...&Chen, Zhiwei.(2013).A Replicating Modified Vaccinia Tiantan Strain Expressing an Avian-Derived Influenza H5N1 Hemagglutinin Induce Broadly Neutralizing Antibodies and Cross-Clade Protective Immunity in Mice.PLOS ONE,8(12).
MLA	Xiao, Haixia,et al."A Replicating Modified Vaccinia Tiantan Strain Expressing an Avian-Derived Influenza H5N1 Hemagglutinin Induce Broadly Neutralizing Antibodies and Cross-Clade Protective Immunity in Mice".PLOS ONE 8.12(2013).