Differential gene expression identified by RNA-Seq and qPCR in two sizes of pearl oyster (Pinctada fucata)

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	Differential gene expression identified by RNA-Seq and qPCR in two sizes of pearl oyster (Pinctada fucata)
	Shi, Y ; He, MX
刊名	GENE
	2014
卷号	538 期号:2 页码:313-322
关键词	Pinctada fucata RNA-Seq Differential gene expression Growth Reproduction Immune system
ISSN号	0378-1119
通讯作者	hmx@scsio.ac.cn
中文摘要	Differential growth of the pearl oyster Pinctada fucata still exists in the aquaculture production. There is no systematic study of the entire transcriptome of differential gene expression in P. fucata in the literature. In this study, high-throughput Illumina/HiSeq (TM) 2000 RNA-Seq was used to examine the differences of gene expression in large (L) and small oysters (S). In total, 74,293 and 76,635 unigenes were generated from Land S oysters, respectively. RT quantitative PCR (qPCR) analysis showed that the differential expression pattern of 19 out of 34 selected genes was consistent with the results of RNA-Seq analysis: 14 genes (11 for growth, 1 for reproduction and 2 for shell formation) were expressed more highly in S,5 genes (1 for growth, 1 for reproduction and 3 for the immune system) were expressed more highly in L; 3 genes associated with the immune system were opposite to it; and no difference was found for the remaining 12 genes. Another 9 shell formation-related genes in L and S were examined by qPCR: 1 gene was expressed more highly in L, 5 genes were expressed more highly in S and no difference was found for the remaining 3 genes. Some genes related to growth and development, shell formation and reproduction were expressed more highly in S compared to L This phenomenon could be explained by "catch-up growth". The results of this study will help toward a comprehensive understanding of the complexity of differential growth between P. fucata individuals and provide valuable information for future research. (C) 2014 Elsevier B.V. All rights reserved.
学科主题	Genetics & Heredity
收录类别	SCI
资助信息	National Science and Technology Program of China [2012AA10A410]; Funds of Knowledge Innovation Program of the Chinese Academy of Sciences [ZCX2-EW-Q21]; Joint Program of NSFC-Guangdong [U0831001]; Marine Fishery Science and Technology Promotion Program of Guangdong Province, China [A201201A05]
原文出处	ELSEVIER SCIENCE BV
WOS记录号	WOS:000333789000014
公开日期	2014-12-11
内容类型	期刊论文
源URL	[http://ir.scsio.ac.cn/handle/344004/10455]
专题	南海海洋研究所_中科院海洋生物资源可持续利用重点实验室
推荐引用方式 GB/T 7714	Shi, Y,He, MX. Differential gene expression identified by RNA-Seq and qPCR in two sizes of pearl oyster (Pinctada fucata)[J]. GENE,2014,538(2):313-322.
APA	Shi, Y,&He, MX.(2014).Differential gene expression identified by RNA-Seq and qPCR in two sizes of pearl oyster (Pinctada fucata).GENE,538(2),313-322.
MLA	Shi, Y,et al."Differential gene expression identified by RNA-Seq and qPCR in two sizes of pearl oyster (Pinctada fucata)".GENE 538.2(2014):313-322.